{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Slavic K"],"funding":["European Research Council"],"pagination":["10403"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4735874"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["Iron is an essential micronutrient but is also highly toxic. In yeast and plant cells, a key detoxifying mechanism involves iron sequestration into intracellular storage compartments, mediated by members of the vacuolar iron-transporter (VIT) family of proteins. Here we study the VIT homologue from the malaria parasites Plasmodium falciparum (PfVIT) and Plasmodium berghei (PbVIT). PfVIT-mediated iron transport in a yeast heterologous expression system is saturable (Km ∼ 14.7 μM), and selective for Fe(2+) over other divalent cations. PbVIT-deficient P. berghei lines (Pbvit(-)) show a reduction in parasite load in both liver and blood stages of infection in mice. Moreover, Pbvit(-) parasites have higher levels of labile iron in blood stages and are more sensitive to increased iron levels in "],"journal":["Nature communications"],"pubmed_title":["A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium."],"pmcid":["PMC4735874"],"funding_grant_id":["311502"],"pubmed_authors":["Vera I","Lahree A","Bouyer G","Krishna S","Hanson KK","Pittman JK","Staines HM","Mota MM","Slavic K"],"additional_accession":[]},"is_claimable":false,"name":"A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium.","description":"Iron is an essential micronutrient but is also highly toxic. In yeast and plant cells, a key detoxifying mechanism involves iron sequestration into intracellular storage compartments, mediated by members of the vacuolar iron-transporter (VIT) family of proteins. Here we study the VIT homologue from the malaria parasites Plasmodium falciparum (PfVIT) and Plasmodium berghei (PbVIT). PfVIT-mediated iron transport in a yeast heterologous expression system is saturable (Km ∼ 14.7 μM), and selective for Fe(2+) over other divalent cations. PbVIT-deficient P. berghei lines (Pbvit(-)) show a reduction in parasite load in both liver and blood stages of infection in mice. Moreover, Pbvit(-) parasites have higher levels of labile iron in blood stages and are more sensitive to increased iron levels in ","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jan","modification":"2025-04-22T19:56:50.197Z","creation":"2019-03-27T02:08:02Z"},"accession":"S-EPMC4735874","cross_references":{"pubmed":["26786069"],"doi":["10.1038/ncomms10403"]}}