biostudies-literatureLaurinyecz BEuropean Molecular Biology OrganizationTÁMOPHungarian Scientific Research FundsNew Szechenyi Plan50169https://www.ebi.ac.uk/biostudies/studies/S-EPMC4736822biostudies-literatureUnknown6(1)Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid composition, because spermatid elongation and individualization requires extensive membrane biosynthesis and remodelling. The bulk of transcriptional activity is completed with the entry of cysts into meiotic division, which makes post-meiotic stages of spermatogenesis very sensitive to even a small reduction in gene products. In this study, we describe the effect of changes in lipid composition during spermatogenesis using a hypomorphic male sterile allele of the Drosophila CDP-DAG synthase (CdsA) gene. We find that the CdsA mutant shows defects in spermatid individualization and enlargement of mitochondria and the axonemal sheath of the spermatids. Furthermore, we could genetically rescue the male sterOpen biologyReduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila.PMC4736822NK1008574.2.6-15/1-2015-00024.2.2.D-15/1/KONV-2015-00164.2.4.A/2-11-1-2012-0001GOP-1.1.1-11-2012-04521825NN111006NF101001Kovacs ALSinka RVedelek VPeter MJuhasz GMaroy PVigh LBalogh GLaurinyecz BfalseReduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila.Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid composition, because spermatid elongation and individualization requires extensive membrane biosynthesis and remodelling. The bulk of transcriptional activity is completed with the entry of cysts into meiotic division, which makes post-meiotic stages of spermatogenesis very sensitive to even a small reduction in gene products. In this study, we describe the effect of changes in lipid composition during spermatogenesis using a hypomorphic male sterile allele of the Drosophila CDP-DAG synthase (CdsA) gene. We find that the CdsA mutant shows defects in spermatid individualization and enlargement of mitochondria and the axonemal sheath of the spermatids. Furthermore, we could genetically rescue the male ster2016-01-01T00:00:00Z2016 Jan2025-04-22T19:51:59.489Z2019-03-27T02:08:05ZS-EPMC47368222679124310.1098/rsob.150169