biostudies-literatureUnknown7Symonds EL<h4>Objectives</h4>To compare the performance of a new blood test for colorectal cancer (CRC) to an established fecal immunochemical test (FIT) in a study population with the full range of neoplastic and non-neoplastic pathologies encountered in the colon and rectum.<h4>Methods</h4>Volunteers were asked to complete a FIT prior to colonoscopy. Blood was collected after bowel preparation but prior to colonoscopy, and plasma was assayed for the presence of methylated BCAT1 and IKZF1 DNA using a multiplex real-time PCR assay. Sensitivity and specificity estimates for the blood test were calculated from true- and false-positive rates for neoplasia and compared with FIT at a range of fecal hemoglobin (Hb) concentration positivity thresholds.<h4>Results</h4>In total, 1,381 volunteers (median age Clinical and translational gastroenterologye137https://www.ebi.ac.uk/biostudies/studies/S-EPMC4737873biostudies-literatureA Blood Test for Methylated BCAT1 and IKZF1 vs. a Fecal Immunochemical Test for Detection of Colorectal Neoplasia.PMC4737873Pedersen SKCole SRYoung GPSymonds ELGopalsamy GMurray DHMangira DBaker RTGaur SLaPointe LCfalseA Blood Test for Methylated BCAT1 and IKZF1 vs. a Fecal Immunochemical Test for Detection of Colorectal Neoplasia.<h4>Objectives</h4>To compare the performance of a new blood test for colorectal cancer (CRC) to an established fecal immunochemical test (FIT) in a study population with the full range of neoplastic and non-neoplastic pathologies encountered in the colon and rectum.<h4>Methods</h4>Volunteers were asked to complete a FIT prior to colonoscopy. Blood was collected after bowel preparation but prior to colonoscopy, and plasma was assayed for the presence of methylated BCAT1 and IKZF1 DNA using a multiplex real-time PCR assay. Sensitivity and specificity estimates for the blood test were calculated from true- and false-positive rates for neoplasia and compared with FIT at a range of fecal hemoglobin (Hb) concentration positivity thresholds.<h4>Results</h4>In total, 1,381 volunteers (median age 2016-01-01T00:00:00Z2016 Jan2025-04-04T14:06:25.846Z2019-03-27T02:08:09ZS-EPMC47378732676512510.1038/ctg.2015.67