biostudies-literatureHishimoto ANCRR NIH HHSNIDA NIH HHSNCI NIH HHSNIHAlbert Einstein College of Medicine415-420https://www.ebi.ac.uk/biostudies/studies/S-EPMC4744103biostudies-literatureUnknown79(5)Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) (MALDI-IMS) provides a technical means for simultaneous analysis of precise anatomic localization and regulation of peptides. We explored the technical capability of matrix-assisted laser desorption ionization mass spectrometry for characterization of peptidomic regulation by an addictive substance along two distinct projection systems in the mouse striatum. The spatial expression patterns of substance P and proenkephalin, marker neuropeptides of two distinct striatal projection neurons, were negatively correlated at baseline. We detected 768 mass/charge (m/z) peaks whose expression levels were mostly negatively and positively correlated with expression levels of substance P and proenkephalin A (amino acidsBiological psychiatryMolecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units.PMC4744103S10 RR0251281S10RR029398P30 CA013330R01 DA024330R01DA024330S10 RR0293981S10RR025128Aguilan JTNishi AKang GAngeletti RHLim JHishimoto ANomaru HYe KNieves EHiroi NfalseMolecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units.Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) (MALDI-IMS) provides a technical means for simultaneous analysis of precise anatomic localization and regulation of peptides. We explored the technical capability of matrix-assisted laser desorption ionization mass spectrometry for characterization of peptidomic regulation by an addictive substance along two distinct projection systems in the mouse striatum. The spatial expression patterns of substance P and proenkephalin, marker neuropeptides of two distinct striatal projection neurons, were negatively correlated at baseline. We detected 768 mass/charge (m/z) peaks whose expression levels were mostly negatively and positively correlated with expression levels of substance P and proenkephalin A (amino acids2016-01-01T00:00:00Z2016 Mar2025-04-21T19:26:20.703Z2019-03-27T02:08:36ZS-EPMC47441032652023910.1016/j.biopsych.2015.09.012