{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cordeiro-Stone M"],"funding":["Curriculum in Toxicology","NIEHS NIH HHS","Environmental Pathology","U.S. Public Health Service","NCI NIH HHS"],"pagination":["68-80"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4745347"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["29(1)"],"pubmed_abstract":["The objective of this study was to assess potential functional attenuation or inactivation of the intra-S checkpoint during melanoma development. Proliferating cultures of skin melanocytes, fibroblasts, and melanoma cell lines were exposed to increasing fluences of UVC and intra-S checkpoint responses were quantified. Melanocytes displayed stereotypic intra-S checkpoint responses to UVC qualitatively and quantitatively equivalent to those previously demonstrated in skin fibroblasts. In comparison with fibroblasts, primary melanocytes displayed reduced UVC-induced inhibition of DNA strand growth and enhanced degradation of p21Waf1 after UVC, suggestive of enhanced bypass of UVC-induced DNA photoproducts. All nine melanoma cell lines examined, including those with activating mutations in BRAF or NRAS oncogenes, also displayed proficiency in activation of the intra-S checkpoint in response to UVC irradiation. The results indicate that bypass of oncogene-induced senescence during melanoma development was not associated with inactivation of the intra-S checkpoint response to UVC-induced DNA replication stress."],"journal":["Pigment cell & melanoma research"],"pubmed_title":["Effective intra-S checkpoint responses to UVC in primary human melanocytes and melanoma cell lines."],"pmcid":["PMC4745347"],"funding_grant_id":["P30-CA16086","P30 CA016086","T32 ES007126","T32 ES007017","P30 ES10126","P01 ES014635","P30 ES010126","RO1 ES015856","R01 ES015856"],"pubmed_authors":["Rao S","Simpson DA","McNulty JJ","Mitchell DL","Cordeiro-Stone M","Zhou Y","Gibbs-Flournoy E","Ibrahim JG","Chastain PD","Sproul CD","Kaufmann WK","Carson C","Thomas NE"],"additional_accession":[]},"is_claimable":false,"name":"Effective intra-S checkpoint responses to UVC in primary human melanocytes and melanoma cell lines.","description":"The objective of this study was to assess potential functional attenuation or inactivation of the intra-S checkpoint during melanoma development. Proliferating cultures of skin melanocytes, fibroblasts, and melanoma cell lines were exposed to increasing fluences of UVC and intra-S checkpoint responses were quantified. Melanocytes displayed stereotypic intra-S checkpoint responses to UVC qualitatively and quantitatively equivalent to those previously demonstrated in skin fibroblasts. In comparison with fibroblasts, primary melanocytes displayed reduced UVC-induced inhibition of DNA strand growth and enhanced degradation of p21Waf1 after UVC, suggestive of enhanced bypass of UVC-induced DNA photoproducts. All nine melanoma cell lines examined, including those with activating mutations in BRAF or NRAS oncogenes, also displayed proficiency in activation of the intra-S checkpoint in response to UVC irradiation. The results indicate that bypass of oncogene-induced senescence during melanoma development was not associated with inactivation of the intra-S checkpoint response to UVC-induced DNA replication stress.","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jan","modification":"2024-11-09T15:54:35.079Z","creation":"2019-03-27T02:08:42Z"},"accession":"S-EPMC4745347","cross_references":{"pubmed":["26437005"],"doi":["10.1111/pcmr.12426"]}}