biostudies-literatureUnknown12(1)Winerdal MNeonatal brain hypoxic ischemia (HI) often results in long-term motor and cognitive impairments. Post-ischemic inflammation greatly effects outcome and adenosine receptor signaling modulates both HI and immune cell function. Here, we investigated the influence of adenosine A1 receptor deficiency (A1R(-/-)) on key immune cell populations in a neonatal brain HI model. Ten-day-old mice were subjected to HI. Functional outcome was assessed by open locomotion and beam walking test and infarction size evaluated. Flow cytometry was performed on brain-infiltrating cells, and semi-automated analysis of flow cytometric data was applied. A1R(-/-) mice displayed larger infarctions (+33%, p < 0.05) and performed worse in beam walking tests (44% more mistakes, p < 0.05) than wild-type (WT) mice. MyeloidPurinergic signalling89-101https://www.ebi.ac.uk/biostudies/studies/S-EPMC4749537biostudies-literatureAdenosine A1 receptors contribute to immune regulation after neonatal hypoxic ischemic brain injury.PMC4749537Wang YQFredholm BBWinerdal MWinerdal MEWinqvist OAden UfalseAdenosine A1 receptors contribute to immune regulation after neonatal hypoxic ischemic brain injury.Neonatal brain hypoxic ischemia (HI) often results in long-term motor and cognitive impairments. Post-ischemic inflammation greatly effects outcome and adenosine receptor signaling modulates both HI and immune cell function. Here, we investigated the influence of adenosine A1 receptor deficiency (A1R(-/-)) on key immune cell populations in a neonatal brain HI model. Ten-day-old mice were subjected to HI. Functional outcome was assessed by open locomotion and beam walking test and infarction size evaluated. Flow cytometry was performed on brain-infiltrating cells, and semi-automated analysis of flow cytometric data was applied. A1R(-/-) mice displayed larger infarctions (+33%, p < 0.05) and performed worse in beam walking tests (44% more mistakes, p < 0.05) than wild-type (WT) mice. Myeloid2016-01-01T00:00:00Z2016 Mar2026-05-05T08:33:40.866Z2019-03-27T02:08:56ZS-EPMC47495372660888810.1007/s11302-015-9482-3