biostudies-literatureSeoane-Zonjic PJunta de AndaluciaSeventh Framework ProgMinisterio de Economía y Competitividad148https://www.ebi.ac.uk/biostudies/studies/S-EPMC4769843biostudies-literatureUnknown17<h4>Background</h4>In the era of DNA throughput sequencing, assembling and understanding gymnosperm mega-genomes remains a challenge. Although drafts of three conifer genomes have recently been published, this number is too low to understand the full complexity of conifer genomes. Using techniques focused on specific genes, gene models can be established that can aid in the assembly of gene-rich regions, and this information can be used to compare genomes and understand functional evolution.<h4>Results</h4>In this study, gene capture technology combined with BAC isolation and sequencing was used as an experimental approach to establish de novo gene structures without a reference genome. Probes were designed for 866 maritime pine transcripts to sequence genes captured from genomic DNA. The BMC genomicsEstablishing gene models from the Pinus pinaster genome using gene capture and BAC sequencing.PMC4769843FP-KBBE-2011-5BIO2012-33797P10-CVI-6075Claros MGArrillaga ICanas RABautista RFernandez-Pozo NAvila CGomez-Maldonado JCanovas FMSeoane-Zonjic PfalseEstablishing gene models from the Pinus pinaster genome using gene capture and BAC sequencing.<h4>Background</h4>In the era of DNA throughput sequencing, assembling and understanding gymnosperm mega-genomes remains a challenge. Although drafts of three conifer genomes have recently been published, this number is too low to understand the full complexity of conifer genomes. Using techniques focused on specific genes, gene models can be established that can aid in the assembly of gene-rich regions, and this information can be used to compare genomes and understand functional evolution.<h4>Results</h4>In this study, gene capture technology combined with BAC isolation and sequencing was used as an experimental approach to establish de novo gene structures without a reference genome. Probes were designed for 866 maritime pine transcripts to sequence genes captured from genomic DNA. The 2016-01-01T00:00:00Z2016 Feb2026-07-15T20:25:04.024Z2019-03-27T02:10:03ZS-EPMC47698432692224210.1186/s12864-016-2490-z