{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Panth KM"],"funding":["Dutch Research Council (NWO)"],"pagination":["22198"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4770595"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6"],"pubmed_abstract":["Matrix metalloproteinase-2 (MMP2) is important in tumorigenesis, angiogenesis and tumor invasion. In this study, we investigated if the Cy5-tagged small immuno protein targeting the catalytic domain of human MMP2 (aMMP2-SIP) detects MMP2 in tumors non-invasively. For this purpose, we generated MMP2 expressing (empty vector EV) and knock-down (KD) HT1080, U373 and U87 cells, which were injected subcutaneously in the lateral flank of NMRI-nu mice. Optical imaging (Optix MX2) performed at 0.5, 2, 4, 8, 24 and 48 hour post injection (h.p.i.) of Cy5 tagged aMMP2-SIP, indicated significantly lower tumor to background ratios at both 24 (P = 0.0090) and 48 h.p.i. (P < 0.0001) for the U87 MMP2-KD compared to control tumors. No differences were found for HT1080 and U373 models. U87 MMP2-KD tumors ha"],"journal":["Scientific reports"],"pubmed_title":["In vivo optical imaging of MMP2 immuno protein antibody: tumor uptake is associated with MMP2 activity."],"pmcid":["PMC4770595"],"funding_grant_id":["911-06-003"],"pubmed_authors":["Panth KM","Losen M","Lieuwes NG","Weber M","Lambin P","van den Beucken T","Dubois LJ","Biemans R","Yaromina A"],"additional_accession":[]},"is_claimable":false,"name":"In vivo optical imaging of MMP2 immuno protein antibody: tumor uptake is associated with MMP2 activity.","description":"Matrix metalloproteinase-2 (MMP2) is important in tumorigenesis, angiogenesis and tumor invasion. In this study, we investigated if the Cy5-tagged small immuno protein targeting the catalytic domain of human MMP2 (aMMP2-SIP) detects MMP2 in tumors non-invasively. For this purpose, we generated MMP2 expressing (empty vector EV) and knock-down (KD) HT1080, U373 and U87 cells, which were injected subcutaneously in the lateral flank of NMRI-nu mice. Optical imaging (Optix MX2) performed at 0.5, 2, 4, 8, 24 and 48 hour post injection (h.p.i.) of Cy5 tagged aMMP2-SIP, indicated significantly lower tumor to background ratios at both 24 (P = 0.0090) and 48 h.p.i. (P < 0.0001) for the U87 MMP2-KD compared to control tumors. No differences were found for HT1080 and U373 models. U87 MMP2-KD tumors ha","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Feb","modification":"2025-04-25T19:06:32.041Z","creation":"2019-03-27T02:10:07Z"},"accession":"S-EPMC4770595","cross_references":{"pubmed":["26923459"],"doi":["10.1038/srep22198"]}}