biostudies-literatureAlinezhad SFP7-PEOPLE (PRO-NEST)NCI NIH HHSCancer Foundation Finland sr10https://www.ebi.ac.uk/biostudies/studies/S-EPMC4772680biostudies-literatureUnknown16<h4>Background</h4>The high false negative rates for initial prostate biopsies refer a large number of the men for repeat biopsies each year. Therefore, biomarkers associated with high risk of the presence of malignancy in histologically benign biopsies could provide a tool to discriminate the patients who need repeat biopsy or intensive follow-up from those who do not. Here we examined the diagnostic applicability of alpha-methylacyl CoA racemase (AMACR) and androgen receptor (AR) mRNA expression and AMACR protein levels in benign and cancerous prostatic tissue.<h4>Methods</h4>AMACR and AR mRNA levels were measured with quantitative, reverse-transcription PCR (qRT-PCR) assays in 79 radical prostatectomy (RP) cases (including 69 benign (RP-Be) and 69 cancerous (RP-PCa) samples) and 19 beniBMC urologyGlobal expression of AMACR transcripts predicts risk for prostate cancer - a systematic comparison of AMACR protein and mRNA expression in cancerous and noncancerous prostate.PMC4772680P30 CA008748238278160065140094Taimen PAlinezhad SBostrom PJOchoa NTPettersson KVaananen RMBjartell AVertosick EAfalseGlobal expression of AMACR transcripts predicts risk for prostate cancer - a systematic comparison of AMACR protein and mRNA expression in cancerous and noncancerous prostate.<h4>Background</h4>The high false negative rates for initial prostate biopsies refer a large number of the men for repeat biopsies each year. Therefore, biomarkers associated with high risk of the presence of malignancy in histologically benign biopsies could provide a tool to discriminate the patients who need repeat biopsy or intensive follow-up from those who do not. Here we examined the diagnostic applicability of alpha-methylacyl CoA racemase (AMACR) and androgen receptor (AR) mRNA expression and AMACR protein levels in benign and cancerous prostatic tissue.<h4>Methods</h4>AMACR and AR mRNA levels were measured with quantitative, reverse-transcription PCR (qRT-PCR) assays in 79 radical prostatectomy (RP) cases (including 69 benign (RP-Be) and 69 cancerous (RP-PCa) samples) and 19 beni2016-01-01T00:00:00Z2016 Feb2025-04-04T11:02:52.352Z2019-06-06T15:31:09ZS-EPMC47726802692832310.1186/s12894-016-0128-8