{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Brennan JC"],"funding":["National Institute of Environmental Health Sciences","Division of Agriculture and Natural Resources, University of California","NIEHS NIH HHS","Japan Health and Labour Sciences","University of California, Davis","Japan Food Hygiene Association"],"pagination":["11903-12"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4772899"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["49(19)"],"pubmed_abstract":["The Ah receptor (AhR)-responsive CALUX (chemically activated luciferase expression) cell bioassay is commonly used for rapid screening of samples for the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin), dioxin-like compounds, and AhR agonists/antagonists. By increasing the number of AhR DNA recognition sites (dioxin responsive elements), we previously generated a novel third generation (G3) recombinant AhR-responsive mouse CALUX cell line (H1L7.5c3) with a significantly enhanced response to DLCs compared to existing AhR-CALUX cell bioassays. However, the elevated background luciferase activity of these cells and the absence of comparable G3 cell lines derived from other species have limited their utility for screening purposes. Here, we describe the development and character"],"journal":["Environmental science & technology"],"pubmed_title":["Development of Species-Specific Ah Receptor-Responsive Third Generation CALUX Cell Lines with Enhanced Responsiveness and Improved Detection Limits."],"pmcid":["PMC4772899"],"funding_grant_id":["R01ES007685","R01 ES007685","P42ES04699","T32 ES007058-33","P42 ES004699"],"pubmed_authors":["Tsutsumi T","Fulton MH","He G","Brennan JC","Zhao J","Denison MS","Wirth E"],"additional_accession":[]},"is_claimable":false,"name":"Development of Species-Specific Ah Receptor-Responsive Third Generation CALUX Cell Lines with Enhanced Responsiveness and Improved Detection Limits.","description":"The Ah receptor (AhR)-responsive CALUX (chemically activated luciferase expression) cell bioassay is commonly used for rapid screening of samples for the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin), dioxin-like compounds, and AhR agonists/antagonists. By increasing the number of AhR DNA recognition sites (dioxin responsive elements), we previously generated a novel third generation (G3) recombinant AhR-responsive mouse CALUX cell line (H1L7.5c3) with a significantly enhanced response to DLCs compared to existing AhR-CALUX cell bioassays. However, the elevated background luciferase activity of these cells and the absence of comparable G3 cell lines derived from other species have limited their utility for screening purposes. Here, we describe the development and character","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Oct","modification":"2025-04-04T12:00:12.077Z","creation":"2019-03-27T02:10:13Z"},"accession":"S-EPMC4772899","cross_references":{"pubmed":["26366531"],"doi":["10.1021/acs.est.5b02906"]}}