biostudies-literatureShin YHNIDCR NIH HHSNIH/NIDCRNational Research Foundation of Korea704-10https://www.ebi.ac.uk/biostudies/studies/S-EPMC4775087biostudies-literatureUnknown466(4)Purinergic receptors, particularly type 7 (P2RX7), are involved in apoptotic cell death. However, the expression and function of P2RX7 are suppressed in HSG cells. In the present study, we explored whether P2RX7 function is regulated by epigenetic alteration of the receptors in two different cell lines, HSG cells derived from human submandibular ducts, and A253 cells, originated from human submandibular carcinoma. We discovered that HSG cells expressed all subtypes of purinergic receptors, excluding P2RX7, at the mRNA level. However, treatment of the cells with 5-Aza-CdR, a DNA demethylating agent, increased the mRNA expression levels of P2RX7 in a time-dependent manner. Furthermore, 5-Aza-CdR completely rescued the calcium response induced by P2RX7 agonist BzATP, a response that was absenBiochemical and biophysical research communicationsEpigenetic alteration of the purinergic type 7 receptor in salivary epithelial cells.PMC4775087R01 DE019644DE0196442014050477Shin YHChoi SYPark KChoi SKNamkoong EKim MKim NLee JHCha SfalseEpigenetic alteration of the purinergic type 7 receptor in salivary epithelial cells.Purinergic receptors, particularly type 7 (P2RX7), are involved in apoptotic cell death. However, the expression and function of P2RX7 are suppressed in HSG cells. In the present study, we explored whether P2RX7 function is regulated by epigenetic alteration of the receptors in two different cell lines, HSG cells derived from human submandibular ducts, and A253 cells, originated from human submandibular carcinoma. We discovered that HSG cells expressed all subtypes of purinergic receptors, excluding P2RX7, at the mRNA level. However, treatment of the cells with 5-Aza-CdR, a DNA demethylating agent, increased the mRNA expression levels of P2RX7 in a time-dependent manner. Furthermore, 5-Aza-CdR completely rescued the calcium response induced by P2RX7 agonist BzATP, a response that was absen2015-01-01T00:00:00Z2015 Oct2025-04-19T18:04:57.858Z2019-03-27T02:10:19ZS-EPMC47750872639968510.1016/j.bbrc.2015.09.095