biostudies-literatureLin SState Key Laboratory of Polymer Materials EngineeringNational Natural Science Foundation of China2023-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC4776518biostudies-literatureUnknown113(8)Real-time online detection of trace threat analytes is critical for global sustainability, whereas the key challenge is how to efficiently convert and amplify analyte signals into simple readouts. Here we report an ultrasensitive microfluidic platform incorporated with smart microgel for real-time online detection of trace threat analytes. The microgel can swell responding to specific stimulus in flowing solution, resulting in efficient conversion of the stimulus signal into significantly amplified signal of flow-rate change; thus highly sensitive, fast, and selective detection can be achieved. We demonstrate this by incorporating ion-recognizable microgel for detecting trace Pb(2+), and connecting our platform with pipelines of tap water and wastewater for real-time online Pb(2+) detectioProceedings of the National Academy of Sciences of the United States of AmericaUltrasensitive microchip based on smart microgel for real-time online detection of trace threat analytes.PMC47765182113600621490582sklpme2014-1-01Liu ZLin SYu HRWang WZhang CChu LYJu XJXie RfalseUltrasensitive microchip based on smart microgel for real-time online detection of trace threat analytes.Real-time online detection of trace threat analytes is critical for global sustainability, whereas the key challenge is how to efficiently convert and amplify analyte signals into simple readouts. Here we report an ultrasensitive microfluidic platform incorporated with smart microgel for real-time online detection of trace threat analytes. The microgel can swell responding to specific stimulus in flowing solution, resulting in efficient conversion of the stimulus signal into significantly amplified signal of flow-rate change; thus highly sensitive, fast, and selective detection can be achieved. We demonstrate this by incorporating ion-recognizable microgel for detecting trace Pb(2+), and connecting our platform with pipelines of tap water and wastewater for real-time online Pb(2+) detectio2016-01-01T00:00:00Z2016 Feb2026-05-05T17:38:51.713Z2019-03-27T02:10:26ZS-EPMC47765182685843510.1073/pnas.1518442113