biostudies-literatureHewitson JPAsthma UKAmerican Asthma FoundationNational Institutes of HealthWellcome TrustNational Institute of General Medical SciencesNIGMS NIH HHS157-170https://www.ebi.ac.uk/biostudies/studies/S-EPMC4776704biostudies-literatureUnknown46(3)Glycan molecules from helminth parasites have been associated with diverse biological functions ranging from interactions with neighbouring host cell populations to down-modulation of specific host immunity. Glycoproteins secreted by the intestinal nematode Heligmosomoides polygyrus are of particular interest as the excretory-secretory products (termed HES) of this parasite contain both heat-labile and heat-stable components with immunomodulatory effects. We used MALDI-TOF-MS and LC-MS/MS to analyse the repertoire of N- and O-linked glycans released from Heligmosomoides polygyrus excretory-secretory products by PNGase A and F, β-elimination and hydrazinolysis revealing a broad range of structures including novel methylhexose- and methylfucose-containing glycans. Monoclonal antibodies to twInternational journal for parasitologyNovel O-linked methylated glycan antigens decorate secreted immunodominant glycoproteins from the intestinal nematode Heligmosomoides polygyrus.PMC4776704106122106122/A/14/ZGM62116090281U54 GM062116P41 GM103694SPD-2012-172P41GM10369410-0234van Diepen ASmit CHHokke CHKoeleman CAMcSorley HJNguyen DLMurray JHewitson JPMaizels RMfalseNovel O-linked methylated glycan antigens decorate secreted immunodominant glycoproteins from the intestinal nematode Heligmosomoides polygyrus.Glycan molecules from helminth parasites have been associated with diverse biological functions ranging from interactions with neighbouring host cell populations to down-modulation of specific host immunity. Glycoproteins secreted by the intestinal nematode Heligmosomoides polygyrus are of particular interest as the excretory-secretory products (termed HES) of this parasite contain both heat-labile and heat-stable components with immunomodulatory effects. We used MALDI-TOF-MS and LC-MS/MS to analyse the repertoire of N- and O-linked glycans released from Heligmosomoides polygyrus excretory-secretory products by PNGase A and F, β-elimination and hydrazinolysis revealing a broad range of structures including novel methylhexose- and methylfucose-containing glycans. Monoclonal antibodies to tw2016-01-01T00:00:00Z2016 Mar2025-04-26T09:18:16.606Z2019-03-27T02:10:25ZS-EPMC47767042668839010.1016/j.ijpara.2015.10.004