{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(3)"],"submitter":["Shu Y"],"pubmed_abstract":["Winter damage, especially in northern climates, is a major limitation of the utilization of perennial forages such as alfalfa. Therefore, improving freezing tolerance is imperative in alfalfa genetic breeding. However, freezing tolerance is a complex trait that is determined by many genes. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed small RNA sequencing analysis under cold (4°) and freezing (-8°) stress. The sequencing results revealed that 173 known, and 24 novel miRNAs were expressed, and that the expression of 35 miRNAs was affected by cold and/or freezing stress. Meanwhile, 105 target genes cleaved by these miRNAs were characterized by degradome sequencing. These targets were associated with biological regulation, cellular processes, m"],"journal":["G3 (Bethesda, Md.)"],"pagination":["755-65"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4777136"],"repository":["biostudies-literature"],"pubmed_title":["Genome-Wide Investigation of MicroRNAs and Their Targets in Response to Freezing Stress in Medicago sativa L., Based on High-Throughput Sequencing."],"pmcid":["PMC4777136"],"pubmed_authors":["Li W","Song L","Liu Y","Zhang J","Shu Y","Guo C"],"additional_accession":[]},"is_claimable":false,"name":"Genome-Wide Investigation of MicroRNAs and Their Targets in Response to Freezing Stress in Medicago sativa L., Based on High-Throughput Sequencing.","description":"Winter damage, especially in northern climates, is a major limitation of the utilization of perennial forages such as alfalfa. Therefore, improving freezing tolerance is imperative in alfalfa genetic breeding. However, freezing tolerance is a complex trait that is determined by many genes. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed small RNA sequencing analysis under cold (4°) and freezing (-8°) stress. The sequencing results revealed that 173 known, and 24 novel miRNAs were expressed, and that the expression of 35 miRNAs was affected by cold and/or freezing stress. Meanwhile, 105 target genes cleaved by these miRNAs were characterized by degradome sequencing. These targets were associated with biological regulation, cellular processes, m","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jan","modification":"2026-05-03T13:09:23.419Z","creation":"2019-03-27T02:10:26Z"},"accession":"S-EPMC4777136","cross_references":{"pubmed":["26801649"],"doi":["10.1534/g3.115.025981"]}}