{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hoesl MG"],"funding":["Defense Advanced Research Projects Agency","EU","Einstein Foundation","DFG","Wellcome Trust","National Institute for General Medical Sciences","NIGMS NIH HHS"],"pagination":["10030-4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4782924"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["54(34)"],"pubmed_abstract":["We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20,899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks."],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["Chemical Evolution of a Bacterial Proteome."],"pmcid":["PMC4782924"],"funding_grant_id":["GRK1582","R01 GM022854","103139/Z/13/Z","N66001-12-C-4020","092076","103139","N66001-12-C-4211"],"pubmed_authors":["Peil L","Oehm S","Rinehart J","Soll D","Hoesl MG","Darmon E","Rappsilber J","Durkin P","Aerni HR","Leach D","Budisa N"],"additional_accession":[]},"is_claimable":false,"name":"Chemical Evolution of a Bacterial Proteome.","description":"We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic noncanonical amino acid L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp→[3,2]Tpa substitutions in their proteomes in response to the 20,899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition showed robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks.","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Aug","modification":"2025-04-05T11:57:04.38Z","creation":"2019-03-27T02:10:40Z"},"accession":"S-EPMC4782924","cross_references":{"pubmed":["26136259"],"doi":["10.1002/anie.201502868"]}}