{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Swoboda RK"],"funding":["NCI NIH HHS"],"pagination":["14009"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4782943"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1"],"pubmed_abstract":["Noncoding regions of the genome play an important role in tumorigenesis of cancer. Using expression cloning, we have identified a cytotoxic T lymphocyte (CTL)-defined antigen that recognizes a protein sequence derived from an open reading frame transcribed from the reverse strand in the 3' untranslated region of tRNA isopentenyltransferase 1 (TRIT1). A peptide derived from this open reading frame (ORF) sequence and predicted to bind to HLA-B57, sensitized HLA-B57(+) tumor cells to lysis by CTL793. The peptide also induced a CTL response in peripheral blood mononuclear cells (PBMC) of patient 793 and in two other melanoma patients. The CTL lysed peptide-pulsed HLA-B57(+) target cells and melanoma cells with endogenous antigen expression. The recognition of this antigen is not limited to HLA"],"journal":["Molecular therapy oncolytics"],"pubmed_title":["Antimelanoma CTL recognizes peptides derived from an ORF transcribed from the antisense strand of the 3' untranslated region of TRIT1."],"pmcid":["PMC4782943"],"funding_grant_id":["P30 CA010815","P01 CA114046","P01 CA025874"],"pubmed_authors":["Herlyn M","Somasundaram R","Robbins P","Swoboda RK","Marincola FM","Caputo-Gross L","Herlyn D"],"additional_accession":[]},"is_claimable":false,"name":"Antimelanoma CTL recognizes peptides derived from an ORF transcribed from the antisense strand of the 3' untranslated region of TRIT1.","description":"Noncoding regions of the genome play an important role in tumorigenesis of cancer. Using expression cloning, we have identified a cytotoxic T lymphocyte (CTL)-defined antigen that recognizes a protein sequence derived from an open reading frame transcribed from the reverse strand in the 3' untranslated region of tRNA isopentenyltransferase 1 (TRIT1). A peptide derived from this open reading frame (ORF) sequence and predicted to bind to HLA-B57, sensitized HLA-B57(+) tumor cells to lysis by CTL793. The peptide also induced a CTL response in peripheral blood mononuclear cells (PBMC) of patient 793 and in two other melanoma patients. The CTL lysed peptide-pulsed HLA-B57(+) target cells and melanoma cells with endogenous antigen expression. The recognition of this antigen is not limited to HLA","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015","modification":"2025-04-04T14:23:32.872Z","creation":"2019-03-27T02:10:40Z"},"accession":"S-EPMC4782943","cross_references":{"pubmed":["27119099"],"doi":["10.1038/mto.2014.9"]}}