biostudies-literatureBergmann TNIAID NIH HHSPHS HHS675-89https://www.ebi.ac.uk/biostudies/studies/S-EPMC4783141biostudies-literatureUnknown67(11-12)Here we describe a detailed quantitative peptide-binding motif for the common equine leukocyte antigen (ELA) class I allele Eqca-1*00101, present in roughly 25 % of Thoroughbred horses. We determined a preliminary binding motif by sequencing endogenously bound ligands. Subsequently, a positional scanning combinatorial library (PSCL) was used to further characterize binding specificity and derive a quantitative motif involving aspartic acid in position 2 and hydrophobic residues at the C-terminus. Using this motif, we selected and tested 9- and 10-mer peptides derived from the equine herpesvirus type 1 (EHV-1) proteome for their capacity to bind Eqca-1*00101. PSCL predictions were very efficient, with an receiver operating characteristic (ROC) curve performance of 0.877, and 87 peptides derImmunogeneticsThe common equine class I molecule Eqca-1*00101 (ELA-A3.1) is characterized by narrow peptide binding and T cell epitope repertoires.PMC4783141HHSN272200900042CR01AI112566AI 033993R01 AI112566U19 AI090023HHSN272201400045CR37 AI033993R01 AI033993U19AI090023Osterrieder NBergmann TPeters BSette AHunt DFWriston ATallmadge RSidney JOseroff CAntczak DFShabanowitz JMoore CMiller DHarman RMfalseThe common equine class I molecule Eqca-1*00101 (ELA-A3.1) is characterized by narrow peptide binding and T cell epitope repertoires.Here we describe a detailed quantitative peptide-binding motif for the common equine leukocyte antigen (ELA) class I allele Eqca-1*00101, present in roughly 25 % of Thoroughbred horses. We determined a preliminary binding motif by sequencing endogenously bound ligands. Subsequently, a positional scanning combinatorial library (PSCL) was used to further characterize binding specificity and derive a quantitative motif involving aspartic acid in position 2 and hydrophobic residues at the C-terminus. Using this motif, we selected and tested 9- and 10-mer peptides derived from the equine herpesvirus type 1 (EHV-1) proteome for their capacity to bind Eqca-1*00101. PSCL predictions were very efficient, with an receiver operating characteristic (ROC) curve performance of 0.877, and 87 peptides der2015-01-01T00:00:00Z2015 Nov2025-04-05T11:56:43.914Z2019-03-27T02:10:40ZS-EPMC47831412639924110.1007/s00251-015-0872-z