biostudies-literatureShao QIntramural NIH HHSNIEHS NIH HHSNHLBI NIH HHSNational Natural Science Foundation of China (NSFC)Division of Intramural Research, National Heart, Lung and Blood InstituteChina Scholarship Council (CSC)HHS | NIH | National Institute of Environmental Health Sciences (NIEHS)HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI)H505-15https://www.ebi.ac.uk/biostudies/studies/S-EPMC4796614biostudies-literatureUnknown310(4)Premenopausal women exhibit endogenous cardioprotective signaling mechanisms that are thought to result from the beneficial effects of estrogen, which we have shown to increase protein S-nitrosylation in the heart. S-nitrosylation is a labile protein modification that increases with a number of different forms of cardioprotection, including ischemic preconditioning. Herein, we sought to identify a potential role for protein S-nitrosylation in sex-dependent cardioprotection. We utilized a Langendorff-perfused mouse heart model of ischemia-reperfusion injury with male and female hearts, and S-nitrosylation-resin-assisted capture with liquid chromatography tandem mass spectrometry to identify S-nitrosylated proteins and modification sites. Consistent with previous studies, female hearts exhibAmerican journal of physiology. Heart and circulatory physiologyCharacterization of the sex-dependent myocardial S-nitrosothiol proteome.PMC4796614Z01 HL002065ZIA-HL-002065R00 HL114721R00HL114721R01HL039752R01 HL039752T32ES0741T32 ES007141R01-HL-03975281570390T32-ES-0741ZIAHL002065R00-HL-11472181270374Steenbergen CKohr MJCasin KMMurphy EFallica JShao QfalseCharacterization of the sex-dependent myocardial S-nitrosothiol proteome.Premenopausal women exhibit endogenous cardioprotective signaling mechanisms that are thought to result from the beneficial effects of estrogen, which we have shown to increase protein S-nitrosylation in the heart. S-nitrosylation is a labile protein modification that increases with a number of different forms of cardioprotection, including ischemic preconditioning. Herein, we sought to identify a potential role for protein S-nitrosylation in sex-dependent cardioprotection. We utilized a Langendorff-perfused mouse heart model of ischemia-reperfusion injury with male and female hearts, and S-nitrosylation-resin-assisted capture with liquid chromatography tandem mass spectrometry to identify S-nitrosylated proteins and modification sites. Consistent with previous studies, female hearts exhib2016-01-01T00:00:00Z2016 Feb2026-05-05T06:27:51.851Z2019-03-27T02:11:22ZS-EPMC47966142670214310.1152/ajpheart.00681.2015