{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(2)"],"submitter":["Szabat M"],"pubmed_abstract":["This is the first report to provide comprehensive thermodynamic and structural data concerning duplex, hairpin, quadruplex and i-motif structures in β-L-RNA series. Herein we confirm that, within the limits of experimental error, the thermodynamic stability of enantiomeric structural motifs is the same as that of naturally occurring D-RNA counterparts. In addition, formation of D-RNA/L-RNA heterochiral duplexes is also observed; however, their thermodynamic stability is significantly reduced in reference to homochiral D-RNA duplexes. The presence of three locked nucleic acid (LNA) residues within the D-RNA strand diminishes the negative effect of the enantiomeric, complementary L-RNA strand in the formation of heterochiral RNA duplexes. Similar behavior is also observed for heterochiral LNA-2'-O-methyl-D-RNA/L-RNA duplexes. The formation of heterochiral duplexes was confirmed by 1H NMR spectroscopy. The CD curves of homochiral L-RNA structural motifs are always reversed, whereas CD curves of heterochiral duplexes present individual features dependent on the composition of chiral strands."],"journal":["PloS one"],"pagination":["e0149478"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4801053"],"repository":["biostudies-literature"],"pubmed_title":["Thermodynamic Features of Structural Motifs Formed by β-L-RNA."],"pmcid":["PMC4801053"],"pubmed_authors":["Pasternak A","Kotkowiak W","Gudanis D","Szabat M","Kierzek R","Gdaniec Z"],"additional_accession":[]},"is_claimable":false,"name":"Thermodynamic Features of Structural Motifs Formed by β-L-RNA.","description":"This is the first report to provide comprehensive thermodynamic and structural data concerning duplex, hairpin, quadruplex and i-motif structures in β-L-RNA series. Herein we confirm that, within the limits of experimental error, the thermodynamic stability of enantiomeric structural motifs is the same as that of naturally occurring D-RNA counterparts. In addition, formation of D-RNA/L-RNA heterochiral duplexes is also observed; however, their thermodynamic stability is significantly reduced in reference to homochiral D-RNA duplexes. The presence of three locked nucleic acid (LNA) residues within the D-RNA strand diminishes the negative effect of the enantiomeric, complementary L-RNA strand in the formation of heterochiral RNA duplexes. Similar behavior is also observed for heterochiral LNA-2'-O-methyl-D-RNA/L-RNA duplexes. The formation of heterochiral duplexes was confirmed by 1H NMR spectroscopy. The CD curves of homochiral L-RNA structural motifs are always reversed, whereas CD curves of heterochiral duplexes present individual features dependent on the composition of chiral strands.","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2025-04-26T01:50:39.192Z","creation":"2019-03-26T22:53:38Z"},"accession":"S-EPMC4801053","cross_references":{"pubmed":["26908023"],"doi":["10.1371/journal.pone.0149478"]}}