biostudies-literatureLi XNEI NIH HHSe0151644https://www.ebi.ac.uk/biostudies/studies/S-EPMC4807090biostudies-literatureUnknown11(3)<h4>Background and purpose</h4>The intraocular pressure (IOP)-lowering and side effects in response to different prostaglandin F2α analogues can be variable, but, the underlying basis for this difference remains unknown. This study investigated the differential changes of cellular proteins relevant to IOP-lowering effects of latanoprost and bimatoprost.<h4>Methods</h4>The human T lymphoblast (MOLT-3) cell line and immortalized human trabecular meshwork (iHTM) cells were studied by quantitative PCR and by immunofluorescence after treatment with either latanoprost or bimatoprost. New Zealand white rabbit eyes were treated topically with each agent and, following euthanasia, anterior segment tissues were studied with immunostaining.<h4>Results</h4>In cultured MOLT-3 cells, mRNA expression of PloS oneEffects of Latanoprost and Bimatoprost on the Expression of Molecules Relevant to Ocular Inflow and Outflow Pathways.PMC4807090P30 EY016665Liu XLi XHe FCai SCao JFan NKaufman PLWang YGabelt BTfalseEffects of Latanoprost and Bimatoprost on the Expression of Molecules Relevant to Ocular Inflow and Outflow Pathways.<h4>Background and purpose</h4>The intraocular pressure (IOP)-lowering and side effects in response to different prostaglandin F2α analogues can be variable, but, the underlying basis for this difference remains unknown. This study investigated the differential changes of cellular proteins relevant to IOP-lowering effects of latanoprost and bimatoprost.<h4>Methods</h4>The human T lymphoblast (MOLT-3) cell line and immortalized human trabecular meshwork (iHTM) cells were studied by quantitative PCR and by immunofluorescence after treatment with either latanoprost or bimatoprost. New Zealand white rabbit eyes were treated topically with each agent and, following euthanasia, anterior segment tissues were studied with immunostaining.<h4>Results</h4>In cultured MOLT-3 cells, mRNA expression of 2016-01-01T00:00:00Z20162025-04-04T20:13:10.73Z2019-03-26T22:53:48ZS-EPMC48070902701123410.1371/journal.pone.0151644