biostudies-literatureAndrijauskaite KNCI NIH HHS360-7https://www.ebi.ac.uk/biostudies/studies/S-EPMC4807400biostudies-literatureUnknown22(7)The ability to genetically modify T cells is a critical component to many immunotherapeutic strategies and research studies. However, the success of these approaches is often limited by transduction efficiency. As retroviral vectors require cell division for integration, transduction efficiency is dependent on the appropriate activation and culture conditions for T cells. Naive CD8(+) T cells, which are quiescent, must be first activated to induce cell division to allow genetic modification. To optimize this process, we activated mouse T cells with a panel of different cytokines, including interleukin-2 (IL-2), IL-4, IL-6, IL-7, IL-12, IL-15 and IL-23, known to act on T cells. After activation, cytokines were removed, and activated T cells were retrovirally transduced. We found that IL-12 Cancer gene therapyIL-12 conditioning improves retrovirally mediated transduction efficiency of CD8+ T cells.PMC4807400P30 CA138313P30CA138313P01 CA154778P01CA54778-01Cole DJSuriano SKochenderfer JNMoxley KMLi MMehrotra SCloud CASalem MLKesarwani PPaulos CMAndrijauskaite KRubinstein MPStefanik LSGarrett-Mayer EfalseIL-12 conditioning improves retrovirally mediated transduction efficiency of CD8+ T cells.The ability to genetically modify T cells is a critical component to many immunotherapeutic strategies and research studies. However, the success of these approaches is often limited by transduction efficiency. As retroviral vectors require cell division for integration, transduction efficiency is dependent on the appropriate activation and culture conditions for T cells. Naive CD8(+) T cells, which are quiescent, must be first activated to induce cell division to allow genetic modification. To optimize this process, we activated mouse T cells with a panel of different cytokines, including interleukin-2 (IL-2), IL-4, IL-6, IL-7, IL-12, IL-15 and IL-23, known to act on T cells. After activation, cytokines were removed, and activated T cells were retrovirally transduced. We found that IL-12 2015-01-01T00:00:00Z2015 Jul2025-04-19T01:25:27.476Z2019-03-27T03:10:16ZS-EPMC48074002618291210.1038/cgt.2015.28