{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Whitten MM"],"funding":["Bill and Melinda Gates Foundation","Biotechnology and Biological Sciences Research Council"],"pagination":["20160042"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4810840"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["283(1825)"],"pubmed_abstract":["RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were "],"journal":["Proceedings. Biological sciences"],"pubmed_title":["Symbiont-mediated RNA interference in insects."],"pmcid":["PMC4810840"],"funding_grant_id":["BB/G024154/1","OPP1068514"],"pubmed_authors":["Dyson PJ","Bodger OG","Del Sol R","Evans MC","Whitten MM","Mitchell JJ","Facey PD","Fernandez-Martinez LT"],"additional_accession":[]},"is_claimable":false,"name":"Symbiont-mediated RNA interference in insects.","description":"RNA interference (RNAi) methods for insects are often limited by problems with double-stranded (ds) RNA delivery, which restricts reverse genetics studies and the development of RNAi-based biocides. We therefore delegated to insect symbiotic bacteria the task of: (i) constitutive dsRNA synthesis and (ii) trauma-free delivery. RNaseIII-deficient, dsRNA-expressing bacterial strains were created from the symbionts of two very diverse pest species: a long-lived blood-sucking bug, Rhodnius prolixus, and a short-lived globally invasive polyphagous agricultural pest, western flower thrips (Frankliniella occidentalis). When ingested, the manipulated bacteria colonized the insects, successfully competed with the wild-type microflora, and sustainably mediated systemic knockdown phenotypes that were ","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Feb","modification":"2026-05-05T11:45:51.534Z","creation":"2019-03-26T22:42:36Z"},"accession":"S-EPMC4810840","cross_references":{"pubmed":["26911963"],"doi":["10.1098/rspb.2016.0042"]}}