biostudies-literatureJeyabal PAmerican Heart AssociationNHLBI NIH HHSNational Institutes of Health423-9https://www.ebi.ac.uk/biostudies/studies/S-EPMC4818978biostudies-literatureUnknown471(4)Diabetic cardiomyopathy is a common complication in patients with diabetes and is associated with underlying chronic inflammation and cardiac cell death, subsequently leading to heart failure (HF). ELAV-like protein 1 (ELAVL1) plays a critical role in the progression of inflammation and HF. However the role of ELAVL-1 in inflammation induced cardiac cell death (pyroptosis) under hyperglycemic condition remains elusive. Our data demonstrates that ELAVL1 expression augmented with a concomitant increase in caspase-1 and IL-1 beta expression in human hearts and human ventricular cardiomyocytes under hyperglycemic condition. Furthermore, ELAVL1 knockdown abrogates TNF-α induced canonical pyroptosis via NLRP3, caspase-1 and IL-1beta suppression. Bioinformatics analysis and target validation assaBiochemical and biophysical research communicationsMicroRNA-9 inhibits hyperglycemia-induced pyroptosis in human ventricular cardiomyocytes by targeting ELAVL1.PMC4818978HL091983P01 HL108795R01 HL126186R01 HL095874R01 HL105597HL108806R01 HL11672915POST25710392R01 HL091983R01 HL053354HL053354HL108795P01 HL108806R37 HL0533541R01HL11672925860041Thandavarayan RAJeyabal PKrishnamurthy PJoladarashi DKrishnamurthy SSuresh Babu SBhimaraj AYouker KAKishore RfalseMicroRNA-9 inhibits hyperglycemia-induced pyroptosis in human ventricular cardiomyocytes by targeting ELAVL1.Diabetic cardiomyopathy is a common complication in patients with diabetes and is associated with underlying chronic inflammation and cardiac cell death, subsequently leading to heart failure (HF). ELAV-like protein 1 (ELAVL1) plays a critical role in the progression of inflammation and HF. However the role of ELAVL-1 in inflammation induced cardiac cell death (pyroptosis) under hyperglycemic condition remains elusive. Our data demonstrates that ELAVL1 expression augmented with a concomitant increase in caspase-1 and IL-1 beta expression in human hearts and human ventricular cardiomyocytes under hyperglycemic condition. Furthermore, ELAVL1 knockdown abrogates TNF-α induced canonical pyroptosis via NLRP3, caspase-1 and IL-1beta suppression. Bioinformatics analysis and target validation assa2016-01-01T00:00:00Z2016 Mar2025-04-04T00:12:37.113Z2019-03-27T03:11:04ZS-EPMC48189782689879710.1016/j.bbrc.2016.02.065