biostudies-literatureGarrido-Urbani SÅke Wiberg StiftelseVetenskapsrådetInstitut National de la Santé et de la Recherche MédicaleCanadian Institutes of Health Research3-12https://www.ebi.ac.uk/biostudies/studies/S-EPMC4819696biostudies-literatureUnknown590(1)Syntenin has crucial roles in cell adhesion, cell migration and synaptic transmission. Its closely linked postsynaptic density-95, discs large 1, zonula occludens-1 (PDZ) domains typically interact with C-terminal ligands. We profile syntenin PDZ1-2 through proteomic peptide phage display (ProP-PD) using a library that displays C-terminal regions of the human proteome. The protein recognizes a broad range of peptides, with a preference for hydrophobic motifs and has a tendency to recognize cryptic internal ligands. We validate the interaction with nectin-1 through orthogonal assays. The study demonstrates the power of ProP-PD as a complementary approach to uncover interactions of potential biological relevance.FEBS lettersProteomic peptide phage display uncovers novel interactions of the PDZ1-2 supramodule of syntenin.PMC4819696MOP-936843773397C0509201Zimmermann PLopez MLembo FSidhu SSSundell GNGhossoub RArnold RGarrido-Urbani SGarg PKim PMIvarsson YfalseProteomic peptide phage display uncovers novel interactions of the PDZ1-2 supramodule of syntenin.Syntenin has crucial roles in cell adhesion, cell migration and synaptic transmission. Its closely linked postsynaptic density-95, discs large 1, zonula occludens-1 (PDZ) domains typically interact with C-terminal ligands. We profile syntenin PDZ1-2 through proteomic peptide phage display (ProP-PD) using a library that displays C-terminal regions of the human proteome. The protein recognizes a broad range of peptides, with a preference for hydrophobic motifs and has a tendency to recognize cryptic internal ligands. We validate the interaction with nectin-1 through orthogonal assays. The study demonstrates the power of ProP-PD as a complementary approach to uncover interactions of potential biological relevance.2016-01-01T00:00:00Z2016 Jan2026-04-07T17:12:32.797Z2019-03-27T03:11:08ZS-EPMC48196962678746010.1002/1873-3468.12037