biostudies-literatureUnknown7Lee NKTo maintain tissue integrity during epithelial morphogenesis, adherens junctions (AJs) must resist the mechanical stresses exerted by dynamic tissue movements. Junctional stability is dependent on actomyosin contractility within the actin ring. Here we describe a novel function for the axon guidance receptor, Neogenin, as a key component of the actin nucleation machinery governing junctional stability. Loss of Neogenin perturbs AJs and attenuates junctional tension. Neogenin promotes actin nucleation at AJs by recruiting the Wave regulatory complex (WRC) and Arp2/3. A direct interaction between the Neogenin WIRS domain and the WRC is crucial for the spatially restricted recruitment of the WRC to the junction. Thus, we provide the first example of a functional WIRS-WRC interaction in epitheNature communications11082https://www.ebi.ac.uk/biostudies/studies/S-EPMC4821876biostudies-literatureNeogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension.PMC4821876Yap ASWilson NHO'Leary CJFok KWCooper HMMichael MWhite ALee NKCox HLfalseNeogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension.To maintain tissue integrity during epithelial morphogenesis, adherens junctions (AJs) must resist the mechanical stresses exerted by dynamic tissue movements. Junctional stability is dependent on actomyosin contractility within the actin ring. Here we describe a novel function for the axon guidance receptor, Neogenin, as a key component of the actin nucleation machinery governing junctional stability. Loss of Neogenin perturbs AJs and attenuates junctional tension. Neogenin promotes actin nucleation at AJs by recruiting the Wave regulatory complex (WRC) and Arp2/3. A direct interaction between the Neogenin WIRS domain and the WRC is crucial for the spatially restricted recruitment of the WRC to the junction. Thus, we provide the first example of a functional WIRS-WRC interaction in epithe2016-01-01T00:00:00Z2016 Mar2026-05-29T20:40:22.247Z2019-03-27T03:11:17ZS-EPMC48218762702959610.1038/ncomms11082