{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6"],"submitter":["Ebstein F"],"pubmed_abstract":["Proteasome-catalyzed peptide splicing represents an additional catalytic activity of proteasomes contributing to the pool of MHC-class I-presented epitopes. We here biochemically and functionally characterized a new melanoma gp100 derived spliced epitope. We demonstrate that the gp100(mel)47-52/40-42 antigenic peptide is generated in vitro and in cellulo by a not yet described proteasomal condensation reaction. gp100(mel)47-52/40-42 generation is enhanced in the presence of the β5i/LMP7 proteasome-subunit and elicits a peptide-specific CD8(+) T cell response. Importantly, we demonstrate that different gp100(mel)-derived spliced epitopes are generated and presented to CD8(+) T cells with efficacies comparable to non-spliced canonical tumor epitopes and that gp100(mel)-derived spliced epitop"],"journal":["Scientific reports"],"pagination":["24032"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4822137"],"repository":["biostudies-literature"],"pubmed_title":["Proteasomes generate spliced epitopes by two different mechanisms and as efficiently as non-spliced epitopes."],"pmcid":["PMC4822137"],"pubmed_authors":["Textoris-Taube K","Schadendorf D","Henklein P","Van den Eynde BJ","Uckert W","Urban S","Vigneron N","Golnik R","Mishto M","Keller C","Albrecht-Koepke N","Ebstein F","Janek K","Kloetzel PM","Lorenz FK","Schuler-Thurner B","Niewienda A","Lehmann A"],"additional_accession":[]},"is_claimable":false,"name":"Proteasomes generate spliced epitopes by two different mechanisms and as efficiently as non-spliced epitopes.","description":"Proteasome-catalyzed peptide splicing represents an additional catalytic activity of proteasomes contributing to the pool of MHC-class I-presented epitopes. We here biochemically and functionally characterized a new melanoma gp100 derived spliced epitope. We demonstrate that the gp100(mel)47-52/40-42 antigenic peptide is generated in vitro and in cellulo by a not yet described proteasomal condensation reaction. gp100(mel)47-52/40-42 generation is enhanced in the presence of the β5i/LMP7 proteasome-subunit and elicits a peptide-specific CD8(+) T cell response. Importantly, we demonstrate that different gp100(mel)-derived spliced epitopes are generated and presented to CD8(+) T cells with efficacies comparable to non-spliced canonical tumor epitopes and that gp100(mel)-derived spliced epitop","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Apr","modification":"2025-04-04T23:50:55.556Z","creation":"2019-03-27T03:11:18Z"},"accession":"S-EPMC4822137","cross_references":{"pubmed":["27049119"],"doi":["10.1038/srep24032"]}}