biostudies-literatureUnknown6Ebstein FProteasome-catalyzed peptide splicing represents an additional catalytic activity of proteasomes contributing to the pool of MHC-class I-presented epitopes. We here biochemically and functionally characterized a new melanoma gp100 derived spliced epitope. We demonstrate that the gp100(mel)47-52/40-42 antigenic peptide is generated in vitro and in cellulo by a not yet described proteasomal condensation reaction. gp100(mel)47-52/40-42 generation is enhanced in the presence of the β5i/LMP7 proteasome-subunit and elicits a peptide-specific CD8(+) T cell response. Importantly, we demonstrate that different gp100(mel)-derived spliced epitopes are generated and presented to CD8(+) T cells with efficacies comparable to non-spliced canonical tumor epitopes and that gp100(mel)-derived spliced epitopScientific reports24032https://www.ebi.ac.uk/biostudies/studies/S-EPMC4822137biostudies-literatureProteasomes generate spliced epitopes by two different mechanisms and as efficiently as non-spliced epitopes.PMC4822137Textoris-Taube KSchadendorf DHenklein PVan den Eynde BJUckert WUrban SVigneron NGolnik RMishto MKeller CAlbrecht-Koepke NEbstein FJanek KKloetzel PMLorenz FKSchuler-Thurner BNiewienda ALehmann AfalseProteasomes generate spliced epitopes by two different mechanisms and as efficiently as non-spliced epitopes.Proteasome-catalyzed peptide splicing represents an additional catalytic activity of proteasomes contributing to the pool of MHC-class I-presented epitopes. We here biochemically and functionally characterized a new melanoma gp100 derived spliced epitope. We demonstrate that the gp100(mel)47-52/40-42 antigenic peptide is generated in vitro and in cellulo by a not yet described proteasomal condensation reaction. gp100(mel)47-52/40-42 generation is enhanced in the presence of the β5i/LMP7 proteasome-subunit and elicits a peptide-specific CD8(+) T cell response. Importantly, we demonstrate that different gp100(mel)-derived spliced epitopes are generated and presented to CD8(+) T cells with efficacies comparable to non-spliced canonical tumor epitopes and that gp100(mel)-derived spliced epitop2016-01-01T00:00:00Z2016 Apr2025-04-04T23:50:55.556Z2019-03-27T03:11:18ZS-EPMC48221372704911910.1038/srep24032