{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jia J"],"funding":["Intramural NIH HHS","NHLBI NIH HHS","NINDS NIH HHS","NIGMS NIH HHS"],"pagination":["75-85"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4834246"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["279"],"pubmed_abstract":["<h4>Objective</h4>Cytochrome P450 epoxygenases (CYP) metabolize arachidonic acid to epoxyeicosatrienoic acids (EETs), which exhibit vasodilatory, anti-inflammatory and neuroprotective actions in experimental cerebral ischemia. We evaluated the effect of endothelial-specific CYP overexpression on cerebral blood flow, inflammatory cytokine expression and tissue infarction after focal cerebral ischemia in transgenic mice.<h4>Approach and results</h4>Male and female wild-type and transgenic mice overexpressing either human CYP2J2 or CYP2C8 epoxygenases in vascular endothelium under control of the Tie2 promoter (Tie2-CYP2J2 and Tie2-CYP2C8) were subjected to 60-min middle cerebral artery occlusion (MCAO). Microvascular cortical perfusion was monitored during vascular occlusion and reperfusion u"],"journal":["Experimental neurology"],"pubmed_title":["Sex- and isoform-specific mechanism of neuroprotection by transgenic expression of P450 epoxygenase in vascular endothelium."],"pmcid":["PMC4834246"],"funding_grant_id":["R01GM088199","P01 NS049210","R01 HL093140","R01 GM088199","R01 NS044313","P01NS049210","R01 NS070837","R01HL093140","Z01 ES025034","R01NS070837"],"pubmed_authors":["DeGraff LM","Davis CM","Graves JP","Lee CR","Jia J","Zhang W","Zeldin DC","Alkayed NJ","Jia T","Wang R","Edin ML","Jouihan S","Ronnekleiv O","Bradbury JA","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Sex- and isoform-specific mechanism of neuroprotection by transgenic expression of P450 epoxygenase in vascular endothelium.","description":"<h4>Objective</h4>Cytochrome P450 epoxygenases (CYP) metabolize arachidonic acid to epoxyeicosatrienoic acids (EETs), which exhibit vasodilatory, anti-inflammatory and neuroprotective actions in experimental cerebral ischemia. We evaluated the effect of endothelial-specific CYP overexpression on cerebral blood flow, inflammatory cytokine expression and tissue infarction after focal cerebral ischemia in transgenic mice.<h4>Approach and results</h4>Male and female wild-type and transgenic mice overexpressing either human CYP2J2 or CYP2C8 epoxygenases in vascular endothelium under control of the Tie2 promoter (Tie2-CYP2J2 and Tie2-CYP2C8) were subjected to 60-min middle cerebral artery occlusion (MCAO). Microvascular cortical perfusion was monitored during vascular occlusion and reperfusion u","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 May","modification":"2025-04-04T08:49:47.971Z","creation":"2019-06-06T15:43:00Z"},"accession":"S-EPMC4834246","cross_references":{"pubmed":["26902473"],"doi":["10.1016/j.expneurol.2016.02.016"]}}