{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Srirangan K"],"funding":["Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","Gouvernement du Canada | Networks of Centres of Excellence of Canada","Canada Research Chairs"],"pagination":["2574-2584"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4836414"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["82(9)"],"pubmed_abstract":["To expand the chemical and molecular diversity of biotransformation using whole-cell biocatalysts, we genetically engineered a pathway in Escherichia coli for heterologous production of butanone, an important commodity ketone. First, a 1-propanol-producing E. coli host strain with its sleeping beauty mutase (Sbm) operon being activated was used to increase the pool of propionyl-coenzyme A (propionyl-CoA). Subsequently, molecular heterofusion of propionyl-CoA and acetyl-CoA was conducted to yield 3-ketovaleryl-CoA via a CoA-dependent elongation pathway. Lastly, 3-ketovaleryl-CoA was channeled into the clostridial acetone formation pathway for thioester hydrolysis and subsequent decarboxylation to form butanone. Biochemical, genetic, and metabolic factors affecting relative levels of ketogen"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Engineering Escherichia coli for Microbial Production of Butanone."],"pmcid":["PMC4836414"],"funding_grant_id":["430106-12","950-211471","BioFuelNet Canada"],"pubmed_authors":["Liu X","Bruder M","Srirangan K","Chou CP","Moo-Young M","Akawi L"],"additional_accession":[]},"is_claimable":false,"name":"Engineering Escherichia coli for Microbial Production of Butanone.","description":"To expand the chemical and molecular diversity of biotransformation using whole-cell biocatalysts, we genetically engineered a pathway in Escherichia coli for heterologous production of butanone, an important commodity ketone. First, a 1-propanol-producing E. coli host strain with its sleeping beauty mutase (Sbm) operon being activated was used to increase the pool of propionyl-coenzyme A (propionyl-CoA). Subsequently, molecular heterofusion of propionyl-CoA and acetyl-CoA was conducted to yield 3-ketovaleryl-CoA via a CoA-dependent elongation pathway. Lastly, 3-ketovaleryl-CoA was channeled into the clostridial acetone formation pathway for thioester hydrolysis and subsequent decarboxylation to form butanone. Biochemical, genetic, and metabolic factors affecting relative levels of ketogen","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 May","modification":"2025-04-04T11:41:36.548Z","creation":"2019-03-27T02:11:55Z"},"accession":"S-EPMC4836414","cross_references":{"pubmed":["26896132"],"doi":["10.1128/AEM.03964-15","10.1128/aem.03964-15"]}}