biostudies-literatureSrirangan KGouvernement du Canada | Natural Sciences and Engineering Research Council of CanadaGouvernement du Canada | Networks of Centres of Excellence of CanadaCanada Research Chairs2574-2584https://www.ebi.ac.uk/biostudies/studies/S-EPMC4836414biostudies-literatureUnknown82(9)To expand the chemical and molecular diversity of biotransformation using whole-cell biocatalysts, we genetically engineered a pathway in Escherichia coli for heterologous production of butanone, an important commodity ketone. First, a 1-propanol-producing E. coli host strain with its sleeping beauty mutase (Sbm) operon being activated was used to increase the pool of propionyl-coenzyme A (propionyl-CoA). Subsequently, molecular heterofusion of propionyl-CoA and acetyl-CoA was conducted to yield 3-ketovaleryl-CoA via a CoA-dependent elongation pathway. Lastly, 3-ketovaleryl-CoA was channeled into the clostridial acetone formation pathway for thioester hydrolysis and subsequent decarboxylation to form butanone. Biochemical, genetic, and metabolic factors affecting relative levels of ketogenApplied and environmental microbiologyEngineering Escherichia coli for Microbial Production of Butanone.PMC4836414430106-12950-211471BioFuelNet CanadaLiu XBruder MSrirangan KChou CPMoo-Young MAkawi LfalseEngineering Escherichia coli for Microbial Production of Butanone.To expand the chemical and molecular diversity of biotransformation using whole-cell biocatalysts, we genetically engineered a pathway in Escherichia coli for heterologous production of butanone, an important commodity ketone. First, a 1-propanol-producing E. coli host strain with its sleeping beauty mutase (Sbm) operon being activated was used to increase the pool of propionyl-coenzyme A (propionyl-CoA). Subsequently, molecular heterofusion of propionyl-CoA and acetyl-CoA was conducted to yield 3-ketovaleryl-CoA via a CoA-dependent elongation pathway. Lastly, 3-ketovaleryl-CoA was channeled into the clostridial acetone formation pathway for thioester hydrolysis and subsequent decarboxylation to form butanone. Biochemical, genetic, and metabolic factors affecting relative levels of ketogen2016-01-01T00:00:00Z2016 May2025-04-04T11:41:36.548Z2019-03-27T02:11:55ZS-EPMC48364142689613210.1128/AEM.03964-1510.1128/aem.03964-15