{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6"],"submitter":["Xu S"],"pubmed_abstract":["RNA G-quadruplexes (G4s) play important roles in translational regulation, mRNA processing events and gene expression. Therefore, a fluorescent probe that is capable of efficiently recognizing RNA G-quadruplex structures among other RNA forms is highly desirable. In this study, a water-soluble fluorogenic dye (i.e., Thioflavin T (ThT)) was employed to recognize RNA G-quadruplex structures using UV-Vis absorption spectra, fluorescence spectra and emission lifetime experiments. By stacking on the G-tetrad, the ThT probe exhibited highly specific recognition of RNA G-quadruplex structures with striking fluorescence enhancement compared with other RNA forms. The specific binding demonstrates that ThT is an efficient fluorescence sensor that can distinguish G4 and non-G4 RNA structures."],"journal":["Scientific reports"],"pagination":["24793"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4838840"],"repository":["biostudies-literature"],"pubmed_title":["Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes."],"pmcid":["PMC4838840"],"pubmed_authors":["Liu Y","Yu L","Xiang J","Xu S","Li Q","Guan A","Yang Q","Chen H","Sun H","Tang Y","Wang L","Shi Y"],"additional_accession":[]},"is_claimable":false,"name":"Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes.","description":"RNA G-quadruplexes (G4s) play important roles in translational regulation, mRNA processing events and gene expression. Therefore, a fluorescent probe that is capable of efficiently recognizing RNA G-quadruplex structures among other RNA forms is highly desirable. In this study, a water-soluble fluorogenic dye (i.e., Thioflavin T (ThT)) was employed to recognize RNA G-quadruplex structures using UV-Vis absorption spectra, fluorescence spectra and emission lifetime experiments. By stacking on the G-tetrad, the ThT probe exhibited highly specific recognition of RNA G-quadruplex structures with striking fluorescence enhancement compared with other RNA forms. The specific binding demonstrates that ThT is an efficient fluorescence sensor that can distinguish G4 and non-G4 RNA structures.","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Apr","modification":"2025-04-22T19:58:17.564Z","creation":"2019-03-27T02:12:04Z"},"accession":"S-EPMC4838840","cross_references":{"pubmed":["27098781"],"doi":["10.1038/srep24793"]}}