{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cunningham AL"],"funding":["NIAID NIH HHS","National Center on Minority Health and Health Disparities","NIMHD NIH HHS","U.S. Department of Defense","National Institutes of Health"],"pagination":["e0153402"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4839702"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(4)"],"pubmed_abstract":["M-cells (microfold cells) are thought to be a primary conduit of intestinal antigen trafficking. Using an established neutralizing anti-RANKL (Receptor Activator of NF-κB Ligand) antibody treatment to transiently deplete M-cells in vivo, we sought to determine whether intestinal M-cells were required for the effective induction of protective immunity following oral vaccination with ΔiglB (a defined live attenuated Francisella novicida mutant). M-cell depleted, ΔiglB-vaccinated mice exhibited increased (but not significant) morbidity and mortality following a subsequent homotypic or heterotypic pulmonary F. tularensis challenge. No significant differences in splenic IFN-γ, IL-2, or IL-17 or serum antibody (IgG1, IgG2a, IgA) production were observed compared to non-depleted, ΔiglB-vaccinated"],"journal":["PloS one"],"pubmed_title":["M-Cells Contribute to the Entry of an Oral Vaccine but Are Not Essential for the Subsequent Induction of Protective Immunity against Francisella tularensis."],"pmcid":["PMC4839702"],"funding_grant_id":["T32AI007271","T32 AI007271","G12MD007591","W911NF-11-1-0136","R21 AI114762","G12 MD007591"],"pubmed_authors":["Cunningham AL","Hung CY","Williams IR","Navara CS","Yagita H","Eaves-Pyles TD","Klose KE","Arulanandam BP","Guentzel MN","Yu JJ","Forsthuber TG"],"additional_accession":[]},"is_claimable":false,"name":"M-Cells Contribute to the Entry of an Oral Vaccine but Are Not Essential for the Subsequent Induction of Protective Immunity against Francisella tularensis.","description":"M-cells (microfold cells) are thought to be a primary conduit of intestinal antigen trafficking. Using an established neutralizing anti-RANKL (Receptor Activator of NF-κB Ligand) antibody treatment to transiently deplete M-cells in vivo, we sought to determine whether intestinal M-cells were required for the effective induction of protective immunity following oral vaccination with ΔiglB (a defined live attenuated Francisella novicida mutant). M-cell depleted, ΔiglB-vaccinated mice exhibited increased (but not significant) morbidity and mortality following a subsequent homotypic or heterotypic pulmonary F. tularensis challenge. No significant differences in splenic IFN-γ, IL-2, or IL-17 or serum antibody (IgG1, IgG2a, IgA) production were observed compared to non-depleted, ΔiglB-vaccinated","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2025-04-19T14:01:02.691Z","creation":"2019-03-26T22:53:50Z"},"accession":"S-EPMC4839702","cross_references":{"pubmed":["27100824"],"doi":["10.1371/journal.pone.0153402"]}}