{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ondeck MG"],"funding":["NCI NIH HHS","NIH HHS"],"pagination":["021003"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4844086"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["138(2)"],"pubmed_abstract":["Hyaluronic acid (HA) is a commonly used natural polymer for cell scaffolding. Modification by methacrylate allows it to be polymerized by free radicals via addition of an initiator, e.g., light-sensitive Irgacure, to form a methacrylated hyaluronic acid (MeHA) hydrogel. Light-activated crosslinking can be used to control the degree of polymerization, and sequential polymerization steps allow cells plated onto or in the hydrogel to initially feel a soft and then a stiff matrix. Here, the elastic modulus of MeHA hydrogels was systematically analyzed by atomic force microscopy (AFM) for a number of variables including duration of UV exposure, monomer concentration, and methacrylate functionalization. To determine how cells would respond to a specific two-step polymerization, NIH 3T3 fibroblas"],"journal":["Journal of biomechanical engineering"],"pubmed_title":["Mechanical Characterization of a Dynamic and Tunable Methacrylated Hyaluronic Acid Hydrogel."],"pmcid":["PMC4844086"],"funding_grant_id":["DP2OD006460","R01 CA206880","DP2 OD006460"],"pubmed_authors":["Engler AJ","Ondeck MG"],"additional_accession":[]},"is_claimable":false,"name":"Mechanical Characterization of a Dynamic and Tunable Methacrylated Hyaluronic Acid Hydrogel.","description":"Hyaluronic acid (HA) is a commonly used natural polymer for cell scaffolding. Modification by methacrylate allows it to be polymerized by free radicals via addition of an initiator, e.g., light-sensitive Irgacure, to form a methacrylated hyaluronic acid (MeHA) hydrogel. Light-activated crosslinking can be used to control the degree of polymerization, and sequential polymerization steps allow cells plated onto or in the hydrogel to initially feel a soft and then a stiff matrix. Here, the elastic modulus of MeHA hydrogels was systematically analyzed by atomic force microscopy (AFM) for a number of variables including duration of UV exposure, monomer concentration, and methacrylate functionalization. To determine how cells would respond to a specific two-step polymerization, NIH 3T3 fibroblas","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Feb","modification":"2025-04-05T15:52:33.62Z","creation":"2019-03-27T02:12:20Z"},"accession":"S-EPMC4844086","cross_references":{"pubmed":["26746491"],"doi":["10.1115/1.4032429"]}}