{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kolind M"],"funding":["Perpetual Foundation","Ramaciotti Foundation","Cancer Institute New South Wales Research Equipment","NHMRC","NIH/NIAMS","Sydney Medical School Research Infrastructure Major Equipment Scheme","NIAMS NIH HHS","Ian Potter Foundation","Australian National Health and Medical Research Council"],"pagination":["53-59"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4844190"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["81"],"pubmed_abstract":["To better understand the relative contributions of mesenchymal and endothelial progenitor cells to rhBMP-2 induced bone formation, we examined the distribution of lineage-labeled cells in Tie2-Cre:Ai9 and αSMA-creERT2:Col2.3-GFP:Ai9 reporter mice. Established orthopedic models of ectopic bone formation in the hind limb and spine fusion were employed. Tie2-lineage cells were found extensively in the ectopic bone and spine fusion masses, but co-staining was only seen with tartrate-resistant acid phosphatase (TRAP) activity (osteoclasts) and CD31 immunohistochemistry (vascular endothelial cells), and not alkaline phosphatase (AP) activity (osteoblasts). To further confirm the lack of a functional contribution of Tie2-lineage cells to BMP-induced bone, we developed conditional knockout mice wh"],"journal":["Bone"],"pubmed_title":["Lineage tracking of mesenchymal and endothelial progenitors in BMP-induced bone formation."],"pmcid":["PMC4844190"],"funding_grant_id":["20100508","730","APP1003480","R01 AR055607","10/REG/1-23","AR055607","3037/2010","2009-02759","APP1003478"],"pubmed_authors":["Schindeler A","Matthews BG","Kolind M","Bobyn JD","Aiken A","Kalajzic I","Mikulec K","Little DG"],"additional_accession":[]},"is_claimable":false,"name":"Lineage tracking of mesenchymal and endothelial progenitors in BMP-induced bone formation.","description":"To better understand the relative contributions of mesenchymal and endothelial progenitor cells to rhBMP-2 induced bone formation, we examined the distribution of lineage-labeled cells in Tie2-Cre:Ai9 and αSMA-creERT2:Col2.3-GFP:Ai9 reporter mice. Established orthopedic models of ectopic bone formation in the hind limb and spine fusion were employed. Tie2-lineage cells were found extensively in the ectopic bone and spine fusion masses, but co-staining was only seen with tartrate-resistant acid phosphatase (TRAP) activity (osteoclasts) and CD31 immunohistochemistry (vascular endothelial cells), and not alkaline phosphatase (AP) activity (osteoblasts). To further confirm the lack of a functional contribution of Tie2-lineage cells to BMP-induced bone, we developed conditional knockout mice wh","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Dec","modification":"2025-04-29T10:12:57.28Z","creation":"2019-03-27T02:12:21Z"},"accession":"S-EPMC4844190","cross_references":{"pubmed":["26141839"],"doi":["10.1016/j.bone.2015.06.023"]}}