biostudies-literatureKolind MPerpetual FoundationRamaciotti FoundationCancer Institute New South Wales Research EquipmentNHMRCNIH/NIAMSSydney Medical School Research Infrastructure Major Equipment SchemeNIAMS NIH HHSIan Potter FoundationAustralian National Health and Medical Research Council53-59https://www.ebi.ac.uk/biostudies/studies/S-EPMC4844190biostudies-literatureUnknown81To better understand the relative contributions of mesenchymal and endothelial progenitor cells to rhBMP-2 induced bone formation, we examined the distribution of lineage-labeled cells in Tie2-Cre:Ai9 and αSMA-creERT2:Col2.3-GFP:Ai9 reporter mice. Established orthopedic models of ectopic bone formation in the hind limb and spine fusion were employed. Tie2-lineage cells were found extensively in the ectopic bone and spine fusion masses, but co-staining was only seen with tartrate-resistant acid phosphatase (TRAP) activity (osteoclasts) and CD31 immunohistochemistry (vascular endothelial cells), and not alkaline phosphatase (AP) activity (osteoblasts). To further confirm the lack of a functional contribution of Tie2-lineage cells to BMP-induced bone, we developed conditional knockout mice whBoneLineage tracking of mesenchymal and endothelial progenitors in BMP-induced bone formation.PMC484419020100508730APP1003480R01 AR05560710/REG/1-23AR0556073037/20102009-02759APP1003478Schindeler AMatthews BGKolind MBobyn JDAiken AKalajzic IMikulec KLittle DGfalseLineage tracking of mesenchymal and endothelial progenitors in BMP-induced bone formation.To better understand the relative contributions of mesenchymal and endothelial progenitor cells to rhBMP-2 induced bone formation, we examined the distribution of lineage-labeled cells in Tie2-Cre:Ai9 and αSMA-creERT2:Col2.3-GFP:Ai9 reporter mice. Established orthopedic models of ectopic bone formation in the hind limb and spine fusion were employed. Tie2-lineage cells were found extensively in the ectopic bone and spine fusion masses, but co-staining was only seen with tartrate-resistant acid phosphatase (TRAP) activity (osteoclasts) and CD31 immunohistochemistry (vascular endothelial cells), and not alkaline phosphatase (AP) activity (osteoblasts). To further confirm the lack of a functional contribution of Tie2-lineage cells to BMP-induced bone, we developed conditional knockout mice wh2015-01-01T00:00:00Z2015 Dec2025-04-29T10:12:57.28Z2019-03-27T02:12:21ZS-EPMC48441902614183910.1016/j.bone.2015.06.023