{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kramer S"],"funding":["Wellcome trust","DFG","Spanish Ministerio de Economía e Innovación","Wellcome Trust"],"pagination":["1-5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4850246"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["205(1-2)"],"pubmed_abstract":["Eukaryotic cells have several mRNA quality control checkpoints to avoid the production of aberrant proteins. Intron-containing mRNAs are actively degraded by the nuclear exosome, prevented from nuclear exit and, if these systems fail, degraded by the cytoplasmic NMD machinery. Trypanosomes have only two introns. However, they process mRNAs from long polycistronic precursors by trans-splicing and polycistronic mRNA molecules frequently arise from any missed splice site. Here, we show that RNAi depletion of the trypanosome exosome, but not of the cytoplasmic 5'-3' exoribonuclease XRNA or the NMD helicase UPF1, causes accumulation of oligocistronic mRNAs. We have also revisited the localization of the trypanosome exosome by expressing eYFP-fusion proteins of the exosome subunits RRP44 and RRP"],"journal":["Molecular and biochemical parasitology"],"pubmed_title":["Polycistronic trypanosome mRNAs are a target for the exosome."],"pmcid":["PMC4850246"],"funding_grant_id":["Kr4017/1-2","085956/Z/08/Z","BFU2014-55193-P","Kr4017/1-1"],"pubmed_authors":["Kramer S","Carrington M","Piper S","Estevez A"],"additional_accession":[]},"is_claimable":false,"name":"Polycistronic trypanosome mRNAs are a target for the exosome.","description":"Eukaryotic cells have several mRNA quality control checkpoints to avoid the production of aberrant proteins. Intron-containing mRNAs are actively degraded by the nuclear exosome, prevented from nuclear exit and, if these systems fail, degraded by the cytoplasmic NMD machinery. Trypanosomes have only two introns. However, they process mRNAs from long polycistronic precursors by trans-splicing and polycistronic mRNA molecules frequently arise from any missed splice site. Here, we show that RNAi depletion of the trypanosome exosome, but not of the cytoplasmic 5'-3' exoribonuclease XRNA or the NMD helicase UPF1, causes accumulation of oligocistronic mRNAs. We have also revisited the localization of the trypanosome exosome by expressing eYFP-fusion proteins of the exosome subunits RRP44 and RRP","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jan-Feb","modification":"2025-04-04T07:30:19.074Z","creation":"2019-03-27T02:12:41Z"},"accession":"S-EPMC4850246","cross_references":{"pubmed":["26946399"],"doi":["10.1016/j.molbiopara.2016.02.009"]}}