biostudies-literatureSolovyeva AIgranting program for “Molecular and Cell Biology” of the Presidium of Russian Academy of SciencesSaint Petersburg State University (RU)Russian Foundation for Basic ResearchRussian Science Foundation34https://www.ebi.ac.uk/biostudies/studies/S-EPMC4850635biostudies-literatureUnknown9<h4>Background</h4>Trematodes have a complex life cycle with animal host changes and alternation of parthenogenetic and hermaphrodite generations. The parthenogenetic generation of the worm (rediae) from the first intermediate host Littorina littorea was used for chromosome spreads production. Karyotype description of parasitic flatworm Himasthla elongata Mehlis, 1831 (Digenea: Himasthlidae) based on fluorochrome banding and 18S rDNA mapping.<h4>Results</h4>Chromosome spreads were obtained from cercariae embryos and redial tissue suspensions with high pressure squash method.74.4 % of the analysed spreads contained 12 chromosome pairs (2n = 24). Chromosome classification was performed according to the morphometry and nomenclature published. H. elongata spread chromosomes had a rather bead-lMolecular cytogeneticsKaryotype features of trematode Himasthla elongata.PMC485063515-15-2002611-04-0170001.2.00 9556391.50.1042.201415-04-0185705-04-49156-аSolovyeva AIStefanova VNDemin SIuPodgornaya OIfalseKaryotype features of trematode Himasthla elongata.<h4>Background</h4>Trematodes have a complex life cycle with animal host changes and alternation of parthenogenetic and hermaphrodite generations. The parthenogenetic generation of the worm (rediae) from the first intermediate host Littorina littorea was used for chromosome spreads production. Karyotype description of parasitic flatworm Himasthla elongata Mehlis, 1831 (Digenea: Himasthlidae) based on fluorochrome banding and 18S rDNA mapping.<h4>Results</h4>Chromosome spreads were obtained from cercariae embryos and redial tissue suspensions with high pressure squash method.74.4 % of the analysed spreads contained 12 chromosome pairs (2n = 24). Chromosome classification was performed according to the morphometry and nomenclature published. H. elongata spread chromosomes had a rather bead-l2016-01-01T00:00:00Z20162026-06-14T08:46:06.623Z2019-06-06T15:45:41ZS-EPMC48506352713465510.1186/s13039-016-0246-8