biostudies-literatureUnknown11(1)Schulz MThe major detoxification product in maize roots after 24 h benzoxazolin-2(3H)-one (BOA) exposure was identified as glucoside carbamate resulting from rearrangement of BOA-N-glucoside, but the pathway of N-glucosylation, enzymes involved and the site of synthesis were previously unknown. Assaying whole cell proteins revealed the necessity of H2O2 and Fe(2+) ions for glucoside carbamate production. Peroxidase produced BOA radicals are apparently formed within the extraplastic space of the young maize root. Radicals seem to be the preferred substrate for N-glucosylation, either by direct reaction with glucose or, more likely, the N-glucoside is released by glucanase/glucosidase catalyzed hydrolysis from cell wall components harboring fixed BOA. The processes are accompanied by alterations of Plant signaling & behaviore1119962https://www.ebi.ac.uk/biostudies/studies/S-EPMC4871689biostudies-literatureBenzoxazolinone detoxification by N-Glucosylation: The multi-compartment-network of Zea mays L.PMC4871689Colby TFilary BHennig LHarzen ASicker DDisko USchmidt JSchulz MKuhn SAnders NHofmann DfalseBenzoxazolinone detoxification by N-Glucosylation: The multi-compartment-network of Zea mays L.The major detoxification product in maize roots after 24 h benzoxazolin-2(3H)-one (BOA) exposure was identified as glucoside carbamate resulting from rearrangement of BOA-N-glucoside, but the pathway of N-glucosylation, enzymes involved and the site of synthesis were previously unknown. Assaying whole cell proteins revealed the necessity of H2O2 and Fe(2+) ions for glucoside carbamate production. Peroxidase produced BOA radicals are apparently formed within the extraplastic space of the young maize root. Radicals seem to be the preferred substrate for N-glucosylation, either by direct reaction with glucose or, more likely, the N-glucoside is released by glucanase/glucosidase catalyzed hydrolysis from cell wall components harboring fixed BOA. The processes are accompanied by alterations of 2016-01-01T00:00:00Z20162026-05-30T11:26:51.527Z2026-04-08T07:11:56.625ZS-EPMC48716892664590910.1080/15592324.2015.1119962