biostudies-literatureUnknown6Wang LFMen have worse survival than premenopausal women after intracerebral hemorrhage (ICH). After ICH, overproduction of iron associated with induction of heme oxygenase-1 (HO-1) in brain was observed. Rodent ICH model using ferrous citrate (FC)-infusion into the striatum to simulate iron overload, showed a higher degree of injury severity in males than in females. However, the participation of HO-1 in sex-differences of iron-induced brain injury remains unknown. The present results showed a higher level of HO-1 expression associated with more severe injury in males compared with females after FC-infusion. Estradiol (E2) contributed to lower levels of FC-induced HO-1 expression in females compared with males. Heterozygote ho-1 KO decreased the levels of FC-induced injury severity, histological Scientific reports26358https://www.ebi.ac.uk/biostudies/studies/S-EPMC4873828biostudies-literatureKnockout of ho-1 protects the striatum from ferrous iron-induced injury in a male-specific manner in mice.PMC4873828Yokoyama KKHsu CLin CLHsiao HWWang LFChiang PCChen TYfalseKnockout of ho-1 protects the striatum from ferrous iron-induced injury in a male-specific manner in mice.Men have worse survival than premenopausal women after intracerebral hemorrhage (ICH). After ICH, overproduction of iron associated with induction of heme oxygenase-1 (HO-1) in brain was observed. Rodent ICH model using ferrous citrate (FC)-infusion into the striatum to simulate iron overload, showed a higher degree of injury severity in males than in females. However, the participation of HO-1 in sex-differences of iron-induced brain injury remains unknown. The present results showed a higher level of HO-1 expression associated with more severe injury in males compared with females after FC-infusion. Estradiol (E2) contributed to lower levels of FC-induced HO-1 expression in females compared with males. Heterozygote ho-1 KO decreased the levels of FC-induced injury severity, histological 2016-01-01T00:00:00Z2016 May2026-05-05T14:43:03.643Z2026-04-07T21:46:38.89ZS-EPMC48738282719853710.1038/srep26358