biostudies-literatureHougaard Pedersen SNovo Nordisk FoundationSpar Nord FoundationResearch Foundation of the Capital Region of DenmarkEUROHEADPAINNovo Nordisk FondenCandy&apos;s Foundatione0155039https://www.ebi.ac.uk/biostudies/studies/S-EPMC4877077biostudies-literatureUnknown11(5)<h4>Introduction</h4>Infusion of glyceryl trinitrate (GTN), a donor of nitric oxide, induces immediate headache in humans that in migraineurs is followed by a delayed migraine attack. In order to achieve increased knowledge of mechanisms activated during GTN-infusion this present study aims to investigate transcriptional responses to GTN-infusion in the rat trigeminal ganglia.<h4>Methods</h4>Rats were infused with GTN or vehicle and trigeminal ganglia were isolated either 30 or 90 minutes post infusion. RNA sequencing was used to investigate transcriptomic changes in response to the treatment. Furthermore, we developed a novel method for Gene Set Analysis Of Variance (GSANOVA) to identify gene sets associated with transcriptional changes across time.<h4>Results</h4>15 genes displayed signiPloS oneRNA Sequencing of Trigeminal Ganglia in Rattus Norvegicus after Glyceryl Trinitrate Infusion with Relevance to Migraine.PMC4877077NNF10SA1016550602633Hougaard Pedersen SKrogh ARamachandran RSibbesen JAOlesen JHansen TFElgaard-Christensen RJansen-Olesen IYakimov VMaretty LfalseRNA Sequencing of Trigeminal Ganglia in Rattus Norvegicus after Glyceryl Trinitrate Infusion with Relevance to Migraine.<h4>Introduction</h4>Infusion of glyceryl trinitrate (GTN), a donor of nitric oxide, induces immediate headache in humans that in migraineurs is followed by a delayed migraine attack. In order to achieve increased knowledge of mechanisms activated during GTN-infusion this present study aims to investigate transcriptional responses to GTN-infusion in the rat trigeminal ganglia.<h4>Methods</h4>Rats were infused with GTN or vehicle and trigeminal ganglia were isolated either 30 or 90 minutes post infusion. RNA sequencing was used to investigate transcriptomic changes in response to the treatment. Furthermore, we developed a novel method for Gene Set Analysis Of Variance (GSANOVA) to identify gene sets associated with transcriptional changes across time.<h4>Results</h4>15 genes displayed signi2016-01-01T00:00:00Z20162026-05-30T03:05:23.385Z2019-03-26T22:53:55ZS-EPMC48770772721395010.1371/journal.pone.0155039