{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Opazo F"],"funding":["European Research Council"],"pagination":["e251"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4877450"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4"],"pubmed_abstract":["Aptamers are valuable tools that provide great potential to develop cost-effective diagnostics and therapies in the biomedical field. Here, we report a novel DNA aptamer that folds into an unconventional G-quadruplex structure able to recognize and enter specifically into human Burkitt's lymphoma cells. We further optimized this aptamer to a highly versatile and stable minimized version. The minimized aptamer can be easily equipped with different functionalities like quantum dots, organic dyes, or even a second different aptamer domain yielding a bi-paratopic aptamer. Although the target molecule of the aptamer remains unknown, our microscopy and pharmacological studies revealed that the aptamer hijacks the clathrin-mediated endocytosis pathway for its cellular internalization. We conclude"],"journal":["Molecular therapy. Nucleic acids"],"pubmed_title":["Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer."],"pmcid":["PMC4877450"],"funding_grant_id":["268776"],"pubmed_authors":["Opazo F","Kjems J","Rohrbach F","Wengel J","Mayer G","Hansen L","Eiden L"],"additional_accession":[]},"is_claimable":false,"name":"Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer.","description":"Aptamers are valuable tools that provide great potential to develop cost-effective diagnostics and therapies in the biomedical field. Here, we report a novel DNA aptamer that folds into an unconventional G-quadruplex structure able to recognize and enter specifically into human Burkitt's lymphoma cells. We further optimized this aptamer to a highly versatile and stable minimized version. The minimized aptamer can be easily equipped with different functionalities like quantum dots, organic dyes, or even a second different aptamer domain yielding a bi-paratopic aptamer. Although the target molecule of the aptamer remains unknown, our microscopy and pharmacological studies revealed that the aptamer hijacks the clathrin-mediated endocytosis pathway for its cellular internalization. We conclude","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Sep","modification":"2025-04-19T14:48:01.048Z","creation":"2019-03-27T02:14:22Z"},"accession":"S-EPMC4877450","cross_references":{"pubmed":["26325628"],"doi":["10.1038/mtna.2015.25"]}}