{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kiss DS"],"funding":["KK-UK","NKB","KK-PhD","OTKA"],"pagination":["417-29"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4887559"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["3"],"pubmed_abstract":["In order to measure the activity of neuronal mitochondria, a representative proof of neuronal processes, physiologically relevant mitochondrial samples need to be gained as simply as possible. Existing methods are, however, either for tissue samples of large size and/or homogenous microstructures only, or are not tested for mitochondrial function measurements. In the present article we describe a gradient fractionation method to isolate viable and well-coupled mitochondria from relatively heterogeneous histological microstructures such as the hypothalamus. With this new method, we are able to isolate a sufficient amount of functional mitochondria for determination of respiratory activity, in a short period of time, using affordable equipment. •Verified by electron microscopy, our method se"],"journal":["MethodsX"],"pubmed_title":["Preparation of purified perikaryal and synaptosomal mitochondrial fractions from relatively small hypothalamic brain samples."],"pmcid":["PMC4887559"],"funding_grant_id":["15263","104982","12021","15930","72186","115613"],"pubmed_authors":["Toth I","Sterczer A","Kiss DS","Jocsak G","Bartha T","Zsarnovszky A","Frenyo LV"],"additional_accession":[]},"is_claimable":false,"name":"Preparation of purified perikaryal and synaptosomal mitochondrial fractions from relatively small hypothalamic brain samples.","description":"In order to measure the activity of neuronal mitochondria, a representative proof of neuronal processes, physiologically relevant mitochondrial samples need to be gained as simply as possible. Existing methods are, however, either for tissue samples of large size and/or homogenous microstructures only, or are not tested for mitochondrial function measurements. In the present article we describe a gradient fractionation method to isolate viable and well-coupled mitochondria from relatively heterogeneous histological microstructures such as the hypothalamus. With this new method, we are able to isolate a sufficient amount of functional mitochondria for determination of respiratory activity, in a short period of time, using affordable equipment. •Verified by electron microscopy, our method se","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2025-06-01T01:57:22.535Z","creation":"2025-06-01T01:57:22.535Z"},"accession":"S-EPMC4887559","cross_references":{"pubmed":["27284533"],"doi":["10.1016/j.mex.2016.05.004"]}}