{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["3"],"submitter":["Zhu Y"],"pubmed_abstract":["Single-cell-type proteomics provides the capability to revealing the genomic and proteomics information at cell-level resolution. However, the methodology for this type of research has not been well-developed. This paper reports developing a workflow of laser capture microdissection (LCM) followed by gel-liquid chromatography-tandem mass spectrometry (GeLC-MS/MS)-based proteomics analysis for the identification of proteomes contained in individual cell layers of tomato roots. Thin-sections (~10-μm thick, 10 sections per root tip) were prepared for root tips of tomato germinating seedlings. Epidermal and cortical cells (5000-7000 cells per tissue type) were isolated under a LCM microscope. Proteins were isolated and then separated by SDS-polyacrylamide gel electrophoresis followed by in-gel"],"journal":["Horticulture research"],"pagination":["16026"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4888759"],"repository":["biostudies-literature"],"pubmed_title":["Development of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots."],"pmcid":["PMC4888759"],"pubmed_authors":["Li H","Yang Y","Zhu Y","Bhatti S","Zhou S","Fish T","Thannhauser TW"],"additional_accession":[]},"is_claimable":false,"name":"Development of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots.","description":"Single-cell-type proteomics provides the capability to revealing the genomic and proteomics information at cell-level resolution. However, the methodology for this type of research has not been well-developed. This paper reports developing a workflow of laser capture microdissection (LCM) followed by gel-liquid chromatography-tandem mass spectrometry (GeLC-MS/MS)-based proteomics analysis for the identification of proteomes contained in individual cell layers of tomato roots. Thin-sections (~10-μm thick, 10 sections per root tip) were prepared for root tips of tomato germinating seedlings. Epidermal and cortical cells (5000-7000 cells per tissue type) were isolated under a LCM microscope. Proteins were isolated and then separated by SDS-polyacrylamide gel electrophoresis followed by in-gel","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2025-04-04T14:06:17.621Z","creation":"2019-03-27T02:14:58Z"},"accession":"S-EPMC4888759","cross_references":{"pubmed":["27280026"],"doi":["10.1038/hortres.2016.26"]}}