biostudies-literatureUnknown3Zhu YSingle-cell-type proteomics provides the capability to revealing the genomic and proteomics information at cell-level resolution. However, the methodology for this type of research has not been well-developed. This paper reports developing a workflow of laser capture microdissection (LCM) followed by gel-liquid chromatography-tandem mass spectrometry (GeLC-MS/MS)-based proteomics analysis for the identification of proteomes contained in individual cell layers of tomato roots. Thin-sections (~10-μm thick, 10 sections per root tip) were prepared for root tips of tomato germinating seedlings. Epidermal and cortical cells (5000-7000 cells per tissue type) were isolated under a LCM microscope. Proteins were isolated and then separated by SDS-polyacrylamide gel electrophoresis followed by in-gelHorticulture research16026https://www.ebi.ac.uk/biostudies/studies/S-EPMC4888759biostudies-literatureDevelopment of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots.PMC4888759Li HYang YZhu YBhatti SZhou SFish TThannhauser TWfalseDevelopment of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots.Single-cell-type proteomics provides the capability to revealing the genomic and proteomics information at cell-level resolution. However, the methodology for this type of research has not been well-developed. This paper reports developing a workflow of laser capture microdissection (LCM) followed by gel-liquid chromatography-tandem mass spectrometry (GeLC-MS/MS)-based proteomics analysis for the identification of proteomes contained in individual cell layers of tomato roots. Thin-sections (~10-μm thick, 10 sections per root tip) were prepared for root tips of tomato germinating seedlings. Epidermal and cortical cells (5000-7000 cells per tissue type) were isolated under a LCM microscope. Proteins were isolated and then separated by SDS-polyacrylamide gel electrophoresis followed by in-gel2016-01-01T00:00:00Z20162025-04-04T14:06:17.621Z2019-03-27T02:14:58ZS-EPMC48887592728002610.1038/hortres.2016.26