{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5"],"submitter":["Lavy M"],"funding":["Howard Hughes Medical Institute","NIGMS NIH HHS"],"pubmed_abstract":["The coordinated action of the auxin-sensitive Aux/IAA transcriptional repressors and ARF transcription factors produces complex gene-regulatory networks in plants. Despite their importance, our knowledge of these two protein families is largely based on analysis of stabilized forms of the Aux/IAAs, and studies of a subgroup of ARFs that function as transcriptional activators. To understand how auxin regulates gene expression we generated a Physcomitrella patens line that completely lacks Aux/IAAs. Loss of the repressors causes massive changes in transcription with misregulation of over a third of the annotated genes. Further, we find that the aux/iaa mutant is blind to auxin indicating that auxin regulation of transcription occurs exclusively through Aux/IAA function. We used the aux/iaa mutant as a simplified platform for studies of ARF function and demonstrate that repressing ARFs regulate auxin-induced genes and fine-tune their expression. Further the repressing ARFs coordinate gene induction jointly with activating ARFs and the Aux/IAAs."],"journal":["eLife"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4889330"],"repository":["biostudies-literature"],"pubmed_title":["Constitutive auxin response in Physcomitrella reveals complex interactions between Aux/IAA and ARF proteins."],"pmcid":["PMC4889330"],"funding_grant_id":["R01 GM043644"],"pubmed_authors":["Shain S","Kuo A","Tao S","Estelle M","Lavy M","Prigge MJ","Kirchsteiger K"],"additional_accession":[]},"is_claimable":false,"name":"Constitutive auxin response in Physcomitrella reveals complex interactions between Aux/IAA and ARF proteins.","description":"The coordinated action of the auxin-sensitive Aux/IAA transcriptional repressors and ARF transcription factors produces complex gene-regulatory networks in plants. Despite their importance, our knowledge of these two protein families is largely based on analysis of stabilized forms of the Aux/IAAs, and studies of a subgroup of ARFs that function as transcriptional activators. To understand how auxin regulates gene expression we generated a Physcomitrella patens line that completely lacks Aux/IAAs. Loss of the repressors causes massive changes in transcription with misregulation of over a third of the annotated genes. Further, we find that the aux/iaa mutant is blind to auxin indicating that auxin regulation of transcription occurs exclusively through Aux/IAA function. We used the aux/iaa mutant as a simplified platform for studies of ARF function and demonstrate that repressing ARFs regulate auxin-induced genes and fine-tune their expression. Further the repressing ARFs coordinate gene induction jointly with activating ARFs and the Aux/IAAs.","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jun","modification":"2021-02-19T20:22:14Z","creation":"2019-03-27T02:15:00Z"},"accession":"S-EPMC4889330","cross_references":{"pubmed":["27247276"],"doi":["10.7554/eLife.13325"]}}