{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Suddala KC"],"funding":["NIGMS NIH HHS"],"pagination":["343-73"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4889436"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["549"],"pubmed_abstract":["Riboswitches are structured noncoding RNA elements that control the expression of their embedding messenger RNAs by sensing the intracellular concentration of diverse metabolites. As the name suggests, riboswitches are dynamic in nature so that studying their inherent conformational dynamics and ligand-mediated folding is important for understanding their mechanism of action. Single-molecule fluorescence energy transfer (smFRET) microscopy is a powerful and versatile technique for studying the folding pathways and intra- and intermolecular dynamics of biological macromolecules, especially RNA. The ability of smFRET to monitor intramolecular distances and their temporal evolution make it a particularly insightful tool for probing the structure and dynamics of riboswitches. Here, we detail the general steps for using prism-based total internal reflection fluorescence microscopy for smFRET studies of the structure, dynamics, and ligand-binding mechanisms of riboswitches."],"journal":["Methods in enzymology"],"pubmed_title":["Riboswitch structure and dynamics by smFRET microscopy."],"pmcid":["PMC4889436"],"funding_grant_id":["R01 GM063162","GM062357","GM063162","R01 GM062357"],"pubmed_authors":["Walter NG","Suddala KC"],"additional_accession":[]},"is_claimable":false,"name":"Riboswitch structure and dynamics by smFRET microscopy.","description":"Riboswitches are structured noncoding RNA elements that control the expression of their embedding messenger RNAs by sensing the intracellular concentration of diverse metabolites. As the name suggests, riboswitches are dynamic in nature so that studying their inherent conformational dynamics and ligand-mediated folding is important for understanding their mechanism of action. Single-molecule fluorescence energy transfer (smFRET) microscopy is a powerful and versatile technique for studying the folding pathways and intra- and intermolecular dynamics of biological macromolecules, especially RNA. The ability of smFRET to monitor intramolecular distances and their temporal evolution make it a particularly insightful tool for probing the structure and dynamics of riboswitches. Here, we detail the general steps for using prism-based total internal reflection fluorescence microscopy for smFRET studies of the structure, dynamics, and ligand-binding mechanisms of riboswitches.","dates":{"release":"2014-01-01T00:00:00Z","publication":"2014","modification":"2021-02-19T20:22:19Z","creation":"2019-06-06T15:52:42Z"},"accession":"S-EPMC4889436","cross_references":{"pubmed":["25432756"],"doi":["10.1016/B978-0-12-801122-5.00015-5","10.1016/b978-0-12-801122-5.00015-5"]}}