{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chakrabarti KR"],"funding":["NIAID NIH HHS","NCI NIH HHS"],"pagination":["10486-97"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4891134"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(9)"],"pubmed_abstract":["Free-floating tumor cells located in the blood of cancer patients, known as circulating tumor cells (CTCs), have become key targets for studying metastasis. However, effective strategies to study the free-floating behavior of tumor cells in vitro have been a major barrier limiting the understanding of the functional properties of CTCs. Upon extracellular-matrix (ECM) detachment, breast tumor cells form tubulin-based protrusions known as microtentacles (McTNs) that play a role in the aggregation and re-attachment of tumor cells to increase their metastatic efficiency. In this study, we have designed a strategy to spatially immobilize ECM-detached tumor cells while maintaining their free-floating character. We use polyelectrolyte multilayers deposited on microfluidic substrates to prevent tu"],"journal":["Oncotarget"],"pubmed_title":["Lipid tethering of breast tumor cells enables real-time imaging of free-floating cell dynamics and drug response."],"pmcid":["PMC4891134"],"funding_grant_id":["T32 AI089621","R01-CA154624","F30-CA196075","R01-CA124704","F30 CA196075","R01 CA154624","R01 CA124704","T32 CA154274"],"pubmed_authors":["Jewell CM","Andorko JI","Sooklal EL","Chakrabarti KR","Whipple RA","Zhang P","Martin SS"],"additional_accession":[]},"is_claimable":false,"name":"Lipid tethering of breast tumor cells enables real-time imaging of free-floating cell dynamics and drug response.","description":"Free-floating tumor cells located in the blood of cancer patients, known as circulating tumor cells (CTCs), have become key targets for studying metastasis. However, effective strategies to study the free-floating behavior of tumor cells in vitro have been a major barrier limiting the understanding of the functional properties of CTCs. Upon extracellular-matrix (ECM) detachment, breast tumor cells form tubulin-based protrusions known as microtentacles (McTNs) that play a role in the aggregation and re-attachment of tumor cells to increase their metastatic efficiency. In this study, we have designed a strategy to spatially immobilize ECM-detached tumor cells while maintaining their free-floating character. We use polyelectrolyte multilayers deposited on microfluidic substrates to prevent tu","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Mar","modification":"2025-04-25T19:13:15.48Z","creation":"2019-03-27T02:15:11Z"},"accession":"S-EPMC4891134","cross_references":{"pubmed":["26871289"],"doi":["10.18632/oncotarget.7251"]}}