biostudies-literatureZheng YNCRR NIH HHSNIGMS NIH HHS2516-26https://www.ebi.ac.uk/biostudies/studies/S-EPMC4891189biostudies-literatureUnknown32(8)<h4>Purpose</h4>Recent controversial publications, citing studies purporting to show that P-gp mediates the transport of propranolol, proposed that passive biological membrane transport is negligible. Based on the BDDCS, the extensively metabolized-highly permeable-highly soluble BDDCS class 1 drug, propranolol, shows a high passive permeability at concentrations unrestricted by solubility that can overwhelm any potential transporter effects. Here we reinvestigate the effects of passive diffusion and carrier-mediated transport on S-propranolol.<h4>Methods</h4>Bidirectional permeability and inhibition of efflux transport studies were carried out in MDCK, MDCK-MDR1 and Caco-2 cell lines at different concentrations. Transcellular permeability studies were conducted at different apical pHs in Pharmaceutical researchpH Dependent but not P-gp Dependent Bidirectional Transport Study of S-propranolol: The Importance of Passive Diffusion.PMC4891189U01 GM061390R43 RR031474U19 GM061390RR031474GM061390Zheng YBenet LZChen XOkochi HfalsepH Dependent but not P-gp Dependent Bidirectional Transport Study of S-propranolol: The Importance of Passive Diffusion.<h4>Purpose</h4>Recent controversial publications, citing studies purporting to show that P-gp mediates the transport of propranolol, proposed that passive biological membrane transport is negligible. Based on the BDDCS, the extensively metabolized-highly permeable-highly soluble BDDCS class 1 drug, propranolol, shows a high passive permeability at concentrations unrestricted by solubility that can overwhelm any potential transporter effects. Here we reinvestigate the effects of passive diffusion and carrier-mediated transport on S-propranolol.<h4>Methods</h4>Bidirectional permeability and inhibition of efflux transport studies were carried out in MDCK, MDCK-MDR1 and Caco-2 cell lines at different concentrations. Transcellular permeability studies were conducted at different apical pHs in 2015-01-01T00:00:00Z2015 Aug2025-04-04T02:35:54.176Z2019-03-27T02:15:11ZS-EPMC48911892569034110.1007/s11095-015-1640-3