biostudies-literatureUnknown6Mourino-Alvarez LAortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there is an inexorable deterioration with a poor prognosis; currently there are no therapies capable of modifying disease progression, and aortic valve replacement is the only available treatment. Our goal is to study the progression of calcification by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) and get new insights at molecular level that could help in the understanding of this disease. In this work, we analyzed consecutive slices from aortic valve tissue by MALDI-IMS, to establish the spatial distribution of proteins and peptides directly from the surface of the histological sections. The analysis showed different structures corresponding to regions observed in conScientific reports27106https://www.ebi.ac.uk/biostudies/studies/S-EPMC4891820biostudies-literatureMALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue.PMC4891820Sanchez PLAzkargorta MBarderas MGde la Cuesta FIloro IPinto AFernandez-Aviles FMourino-Alvarez LSastre-Oliva TLopez-Almodovar LFPadial LRAkerstrom FUrreta HEscobes IElortza FfalseMALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue.Aortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there is an inexorable deterioration with a poor prognosis; currently there are no therapies capable of modifying disease progression, and aortic valve replacement is the only available treatment. Our goal is to study the progression of calcification by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) and get new insights at molecular level that could help in the understanding of this disease. In this work, we analyzed consecutive slices from aortic valve tissue by MALDI-IMS, to establish the spatial distribution of proteins and peptides directly from the surface of the histological sections. The analysis showed different structures corresponding to regions observed in con2016-01-01T00:00:00Z2016 Jun2026-05-30T09:10:49.035Z2019-03-27T02:15:15ZS-EPMC48918202725677010.1038/srep27106