{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(3)"],"submitter":["Thieltges F"],"pubmed_abstract":["<h4>Purpose</h4>We developed a surgical method for localized and atraumatic removal of the retinal pigment epithelium (RPE) with a novel instrument.<h4>Methods</h4>Bleb retinal detachments (bRD) were raised with balanced salt solution (BSS) following vitrectomy in 27 rabbits. The RPE was scraped with 3 loop variants (polypropylene [PP], 0.1 mm; PP, 0.06 mm; metal, 0.1 mm) of a custom-made instrument. Stabilization of bRDs with BSS or various concentrations (0.1%-0.5%) of hyaluronic acid (HA) was video analyzed. Perfusion-fixed samples of scraped areas and controls were studied by light and transmission electron microscopy.<h4>Results</h4>The bRDs were sufficiently stabilized by ≥0.25% HA. Using the PP 0.1 mm loop with a single forward/backward stroke, an area of ca. 2.5 × 1.5 mm was nearly"],"journal":["Translational vision science & technology"],"pagination":["11"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4898042"],"repository":["biostudies-literature"],"pubmed_title":["Localized RPE Removal with a Novel Instrument Aided by Viscoelastics in Rabbits."],"pmcid":["PMC4898042"],"pubmed_authors":["Brinken R","Stanzel BV","Liu Z","Somboonthanakij S","Thieltges F","Holz FG","Braun N","Wongsawad W","Herwig M"],"additional_accession":[]},"is_claimable":false,"name":"Localized RPE Removal with a Novel Instrument Aided by Viscoelastics in Rabbits.","description":"<h4>Purpose</h4>We developed a surgical method for localized and atraumatic removal of the retinal pigment epithelium (RPE) with a novel instrument.<h4>Methods</h4>Bleb retinal detachments (bRD) were raised with balanced salt solution (BSS) following vitrectomy in 27 rabbits. The RPE was scraped with 3 loop variants (polypropylene [PP], 0.1 mm; PP, 0.06 mm; metal, 0.1 mm) of a custom-made instrument. Stabilization of bRDs with BSS or various concentrations (0.1%-0.5%) of hyaluronic acid (HA) was video analyzed. Perfusion-fixed samples of scraped areas and controls were studied by light and transmission electron microscopy.<h4>Results</h4>The bRDs were sufficiently stabilized by ≥0.25% HA. Using the PP 0.1 mm loop with a single forward/backward stroke, an area of ca. 2.5 × 1.5 mm was nearly","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jun","modification":"2025-06-01T01:58:19.174Z","creation":"2025-06-01T01:58:19.174Z"},"accession":"S-EPMC4898042","cross_references":{"pubmed":["27294010"],"doi":["10.1167/tvst.5.3.11"]}}